氮离子注入和微波复合诱变选育高产柠檬酸的黑曲霉研究  

Study on the mutation breeding of Aspergillus niger fermented production citric Acid

作　　者：王德培[1] 周婷[2] 张灵燕 杨赢[1] 高年发[1] 

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]工业发酵微生物教育部重点实验室(天津科技大学),天津300457 [3]日照鲁信金禾生化有限公司,山东日照276800

出　　处：《中国酿造》2012年第5期123-127,共5页China Brewing

摘　　要：利用低能离子注入及微波辐射对柠檬酸生产菌进行诱变选育。在30keV能量的氮离子注入以及间隔10s的微波电磁辐射后筛选得到2株产量提高到60%的诱变高产菌,多次传代实验表明菌株遗传稳定性良好,为柠檬酸发酵菌黑曲霉的进一步的育种工作提供了诱变参数和高产出发菌株。The mutagenic effects of Aspergillus niger strains by low Energy ion implantation and microwave radiation had been studied.Through 30keV N＋ Implanting and interval 10s of microwave radiation on citric acid producing bacteria,two high-yield mutant strains were obtained,Their citric acid production ability was increased by about 60% than that of original.The results showed that the mutant strains had high genetic stability.It provided the mutagenic parameters and the high yield strain for the further breeding work of the citric acid fermentation byAspergillus niger.

关 键 词：黑曲霉 柠檬酸 离子注入 复合诱变 

分 类 号：TS201.3[轻工技术与工程—食品科学]