红色单质硒/DOX-淀粉复合颗粒的制备及抗肿瘤协同效果分析  

Synthesis and synergetic anti-tumor effect of Se/DOX-StNP

作　　者：肖苏尧[1] 赵力超[1] 刘晓娟[1] 曾颖[1] 庄濠宇[1] 曹庸[1] 

机构地区：[1]华南农业大学食品学院,广东广州510642

出　　处：《食品工业科技》2012年第12期114-117,共4页Science and Technology of Food Industry

基　　金：国家自然科学基金(31100433);"林业重大公益性行业科研专项"(201104003-03)

摘　　要：合成了红色单质硒-多柔比星-淀粉复合纳米颗粒,并作用于肝癌BEL7404细胞,采用MTT法检测其对细胞的生长抑制情况。将Na2SeO3还原成红色单质硒,然后通过疏水作用结合到装载有多柔比星(DOX)的淀粉纳米颗粒的表面,形成红色单质硒-多柔比星-淀粉复合颗粒(Se/DOX-StNP);采用Zeta-size粒度仪检测其颗粒分布和表面电荷,颗粒直径范围为50～80nm,表面电荷为-13.9mV;透射电镜结果显示具有明显核壳结构;颗粒作用于肝癌BEL7404细胞,其细胞生长抑制率比相当量的红色单质硒和DOX-淀粉纳米颗粒(DOX-StNP)的细胞抑制率总和高出15%,展现了红色单质硒促进DOX抗肿瘤活性的协同效果。结果表明,复合制剂实现不同药物的同时给药,有较好的抗肿瘤效果,这将为抗肿瘤药物的药剂学研究提供新的思路。Drug-loaded Se-StNP with size of 50～80nm was obtained by covertion of red element Se on the surface of DOX-loaded StNP,which had obvious shell-core structure,and the surface charge was-13.9mV.Drug-loaded Se-StNPs were incubated with liver cancer BEL7404 cell,and MTT assay was used to detect the cell growth-inhibition ratio.The result showed that the growth-inhibition ratio of Drug-loaded Se-StNPs was higher by 15% than the summation of growth-inhibition ratios of element Se and DOX-StNP.This indicated that the synergetic anti-tumor effect was achieved by using DOX-loaded Se-StNPs.The possible reasons for the synergetic anti-tumor effect of complex nanoparticles were the follows：the first was that the speed of drug-release was slowed down because of the protection of shell-core structure.The second was that element Se eliminated the oxidated material around cells,and inhibited the speed of tumor cell division.In addition the permeability of cell membrane was improved and was available for drug’s transporting into cell.The final reason was that the negative element Se increased the electronegation of cell membrane and the permeability of cell membrane was thus improved further.

关 键 词：红色单质硒 多柔比星 复合纳米颗粒 抗肿瘤 协同效果 

分 类 号：TS201.2[轻工技术与工程—食品科学]