响应面法优化酶法提取泥鳅鱼油的研究  

Optimization of Enzyme-Assisted Extraction Process for Misgurnus anguillicaudatus Oil by Response Surface Methodology

作　　者：姚东瑞[1,2] 周鸣谦[1,2] 刘云鹤[1] 苏靖昕 

机构地区：[1]淮海工学院海洋学院,连云港222005 [2]江苏省海洋资源开发研究院,连云港222005

出　　处：《中国粮油学报》2012年第6期66-70,共5页Journal of the Chinese Cereals and Oils Association

基　　金：江苏省科技支撑计划(农业)(BE2010399);江苏省产学研联合创新资金项目(BY2010127)

摘　　要：采用响应面法优化酶法提取泥鳅鱼油工艺,并采用GC/MS法分析泥鳅鱼油成分。结果显示:酶法提取泥鳅鱼油的最优工艺条件为酶添加量1.66%、酶解时间2.5 h、液料比2.18∶1、酶解温度56℃,此条件下泥鳅鱼油的平均得率为6.48%;泥鳅鱼油中饱和脂肪酸占27.23%,不饱和脂肪酸占67%左右,其中单不饱和脂肪酸占43.03%,以油酸(28.17%)、棕榈油酸(9.51%)、顺型异油酸(5.35%)为主,多不饱和脂肪酸以亚油酸为主(21.31%),此外还含有亚麻酸与EPA,具有一定的开发利用价值。Enzyme - assisted extraction process for Misgurrtus anguillicaudatus oil was optimized by response surface methodology and the oil was analyzed by GC/MS in this paper. The results showed：the optimized extraction process was enzyme concentration of 1.66%, enzymatic hydrolysis time of 2.5 hours, solvent / solid ratio of 2.18：1 and enzymatic hydrolysis temperature of 56 ~C. Under above conditions ,the average extraction rate was 6.48%. There were saturated fatty acid of 27.23% and unsaturated fatty acid of about 67% in Misgurnus anguillicaudatus oil, in which there were monounsaturated fatty acid of 43.03% and polyunsaturated fatty acid of 21.31%. Monounsaturated fatty acid included oleic acid of 28.17% ,palmitoleic acid of 9.51% and vaccenic acid of 5.35% mainly. Linoleic acid was the main polyunsaturated fatty acid in Misgurnus anguillicaudatus oil. In addition, there were ot -linolenic acid and EPA . also. The Mis^urmL, an,~rl, illica.r~.,t,7.t1~,~ nil ;~ wn^h ,~nl,~;＋; A ,,o;~

关 键 词：泥鳅鱼油 酶解 气相色谱-质谱联用 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]