超高压协同温度处理对烟熏火腿中单增李斯特菌生长预测模型的建立  

Predictive modelling of the recovery of Listeria monocytogenes on cooked smoked ham after high pressure combining with different temperature processing

作　　者：孙新生[1] 徐幸莲[1] 韩衍青[1] 王虎虎[1] 周光宏[1] 

机构地区：[1]教育部肉品加工与质量控制重点实验室,南京农业大学,江苏南京210095

出　　处：《食品工业科技》2012年第13期118-121,126,共5页Science and Technology of Food Industry

基　　金：江苏省科技成果转化专项资金项目(BA2009007);农业行业科技专项资金(200903012)

摘　　要：为了建立超高压协同温度处理烟熏火腿中单增李斯特菌生长预测模型。本研究分别以20、30、40、50℃结合600MPa对接种106cfu/g单增李斯特菌烟熏切片火腿进行5min的超高压处理,4℃贮藏条件下,每10d测定处理后样品中单增李斯特菌的数量。结果表明:当超高压协同处理的温度高于40℃时显著延长了单增李斯特菌在烟熏火腿修复生长的延滞期,所得生长曲线用修正的Gompertz模型进行了拟合,在35、45d进行验证,建立了烟熏火腿中单增李斯特菌在超高压协同温度处理后的生长预测模型,经验证其精确因子(Af)、偏差因子(Bf)、根平均方差(RMSE)和决定系数(R2)均在较好范围内,能较好的模拟单增李斯特菌的生长情况,为低温肉制品中单增李斯特菌的控制提供理论参考。To establish suitable predictive model for the recovery of Listeria monocytogenes on cooked smoked ham after high pressure combining with different temperature processing. Cooked smoked ham, inoculated with 10%fu/g L. Monocytogenes,was subjected to high pressure treatments at 600MPa for 5min combined with 20,30,40 and 50℃respectively,and then stored at 4℃,counts of L. Monocytogenes were determined every 10 days after treatment. It was found that the treatment temperature higher than 40℃ combining with high- pressure efficiently prolong self-repairing growth postponing period of L. Monocytogenes in sliced vacuum- packaged smoked cooked ham. The growth curve of L. Monocytogenes was imitated by modified Gompertz type model and validated at 35 and 45d,respectively,to establish a growth predictive model of L. Monocytogenes in sliced vacuum-packaged smoked cooked ham treated by high pressure and temperature. After validation of the model,the results of Af,Bf,RMSE and R2 were proved to be in a proper range. Consequently,the result suggested that the model could well imitate growth condition of L. Monocytogenes and it could provide a theory basis of L. Monocytogenes control in low-temperature meat product.

关 键 词：超高压 单增李斯特菌 修正的Gompertz模型 烟熏火腿 

分 类 号：TS251.5[轻工技术与工程—农产品加工及贮藏工程]