高产γ-氨基丁酸乳酸菌菌株的筛选及诱变育种研究  

Study on screening and mutation breeding of high-yield γ-aminobuyric acid Lactobacillus

作　　者：吴非[1] 边鑫[2] 李良[1] 吴海波[3] 郭丽[3] 

机构地区：[1]东北农业大学食品学院,哈尔滨150030 [2]国家大豆工程技术研究中心,哈尔滨150050 [3]绥化学院生物与食品工程系,黑龙江绥化152061

出　　处：《东北农业大学学报》2012年第5期6-10,共5页Journal of Northeast Agricultural University

基　　金：黑龙江省自然科学基金(C200942)

摘　　要：以乳酸菌作为出发菌株,将其接入豆浆(大豆水=1 8)进行发酵,根据发酵过程中γ-氨基丁酸(GABA)产量,筛选出GABA的高产乳酸菌菌株,然后利用紫外线对高产菌株进行诱变处理,筛选得到稳定高产GABA突变菌株。结果表明,保加利亚乳杆菌L2为高产GABA乳酸菌菌株,GABA产量达到1.066 g.L-1。对L2进行紫外诱变处理的最佳照射时间为50 s,在此照射时间下,获得高产GABA突变菌株L2-4,其在含有1%L-谷氨酸的改良MRS培养基和豆浆中的GABA产量分别为4.235和1.394 g.L-1,比原菌株的GABA产量分别提高了25.63%和30.77%。Soya-bean milk （soybean： water=1： 8） were fermented by Lactobacillus. High-yield GABA strain was screened according to the y-aminobuyric acid （GABA） accumulation amount during soya-bean milk fermentation. The high-yield GABA stable mutant strain was obtained by ultraviolet mutagenesis. The results showed that Lactobacillus bulgaricus L2 was high-yield GABA strain by the screening of GABA-yield Lactobacillus, GABA yield reached 1.066 g. L1. The optimum mutagenesis time of L2 by ultraviolet was 50 s. In this condition, a high-yield GABA stable mutant strain named L2-4 was obtained, and the GABA yields were 4.235 go L-1 in reformed MRS （containing 1% L-glutamic acid） and 1.394 g · L-1 in soya-bean milk. Compared to the initial strain, GABA yield of mutant strain 25.63% in reformed MRS （containing 1% L-glutamic acid） and 30.77% in soya-bean milk.

关 键 词：Γ-氨基丁酸 乳酸菌 筛选 紫外诱变 高产突变菌株 

分 类 号：S852.6[农业科学—基础兽医学]