小麦粉水溶性阿拉伯木聚糖提取过程中蛋白质脱除方法的研究  

STUDY ON PROTEIN REMOVAL DURING EXTRACTION OF WATER-SOLUBLE ARABINOXYLANS FROM WHEAT FLOUR

作　　者：邢丽艳[1] 苏东民[1] 苏东海[2] 魏雪芹[1] 马四平[1] 李雪[1] 

机构地区：[1]河南工业大学粮油食品学院,河南郑州450001 [2]北京电子科技职业学院生物技术系,北京100029

出　　处：《河南工业大学学报（自然科学版）》2012年第3期24-27,32,共5页Journal of Henan University of Technology：Natural Science Edition

基　　金：国家自然科学基金项目(31071597);北京市属高等学校人才强教计划资助项目

摘　　要：以小麦面粉为原料,采用水提醇沉法提取水溶性阿拉伯木聚糖(WE-AX),比较了硅藻土吸附法和酶法两种方法在WE-AX提取过程中的蛋白脱除效果.结果表明:硅藻土吸附法操作简便、快捷,但蛋白脱除效率偏低;酶法蛋白脱除效率较高,但操作时间长,能耗高,后续处理困难.采用正交试验确定了酶法脱除蛋白的最佳条件为:中性蛋白酶添加量为0.06%(W/V),反应温度为50℃,反应时间为4 h,蛋白质脱除率为76.20%.同时,结合WE-AX整体提取过程中其他环节的蛋白质脱除情况,可使蛋白质脱除率达到99.7%.In this paper, we extracted water-soluble arabinoxylans （WE-AX） from wheat flour by water extraction and ethanol precipitation method, and compared the protein removal effects of diatomite adsorption method and enzyme method during extraction of WE-AX. The results showed that the diatomite adsorption method was simple and fast, but had low protein removal rate; and enzyme method had high protein removal rate, but long operation time, high energy consumption and difficult post-treatment. The optimum protein removal conditions of enzyme method were determined by orthogonal experiments as follows： neutral protease amount 0.06% （W/V）, reaction temperature 50 ~C, and reaction time 4 hours. Under the optimum conditions, the protein removal rate was 76.20%. Since the protein could be removed in other links of the extraction process, the total protein removal rate could reach 99.7%.

关 键 词：小麦粉 水溶性阿拉伯木聚糖 蛋白质脱除 蛋白酶 硅藻土 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]