ε-聚赖氨酸的活性及其应用  

The activity and application of ε-PL

作　　者：宋庆超[1] 张然[1] 贾士儒[1] 

机构地区：[1]工业微生物教育部重点实验室,天津科技大学,天津300457

出　　处：《中国食品添加剂》2012年第3期179-183,共5页China Food Additives

基　　金：国家高技术研究发展计划支持(863)(资助号:2006AA10Z347)

摘　　要：ε-聚赖氨酸(ε-Poly-L-lysine,ε-PL)由放线菌产生,含有25 30个L-赖氨酸残基,其通过L-赖氨酸α-羧基和ε-氨基间形成的酰胺键连接而成。ε-PL是一种具有抑菌功效的阳离子型多肽,抑菌谱广,能够抑制革兰氏阳性菌、革兰氏阴性菌、酵母菌、真菌以及噬菌体。又因其具有稳定性、安全性以及生物可降解性,日本、美国等国家已先后批准其作为食品添加剂使用,但在我国,ε-PL的应用仍处于研发阶段。许多报道都研究了ε-PL的抑菌活性,推测其抑菌机理是ε-PL破坏微生物的细胞膜结构,抑制微生物的呼吸作用,同时抑制蛋白的合成,但其杀菌机理仍不清楚,需要更进一步研究。本文总结了有关ε-PL的抑菌性能和机理及其在食品、医药等领域中的应用进展,并展望了其作为生物防腐剂的应用前景。ε - Poly - L - lysine （ ε - PL）, consisting of 25 - 30 L - lysine residues with a linkage between ε - car- boxyl group and ε - amino group, is produced by actinomycetes. ε - PL is a polycationic peptide, which exhibits antimicrobial activity on both Grampositive and Gram -negative bacteria. Because it is stable, safe and biodegradable, ε - PL is used as a food preservative in Japan and America. However, the application of ε - PL is under developed in China. Many reports have been published on antimicrobial activity of ε - PL. The antimicrobial mechanism of ε - PL may due its ability in destroying the cell membrane of microorganism, thus inhibits their respiration and the composition of protein. However, its sterilization mechanism has not been reported yet. This paper reviews the antimicrobial activity and mechanism of ε - PL, as well as its application in food and medicine. The application prospects of a biological preservative is also discussed.

关 键 词：Ε-聚赖氨酸 生物防腐剂 抑菌性能 安全性 应用 

分 类 号：TS202.3[轻工技术与工程—食品科学]