响应面法优化油菜蜂花粉核苷类成分提取工艺  

Optimization of Nucleosides Extraction from Rape Bee Pollen Using Response Surface Methodology

作　　者：孙丽萍[1] 黄兰[1,2] 徐响[1] 庞杰[2] 何伟[1,2] 穆雪峰[1,2] 沈新锋[1,2] 

机构地区：[1]中国农业科学院蜜蜂研究所,北京100093 [2]福建农林大学食品科学学院,福建福州350002

出　　处：《食品科学》2012年第12期83-88,共6页Food Science

基　　金："十二五"国家科技支撑计划项目(2011BAD33B04);国家现代农业(蜂)产业技术体系建设专项(CARS-45KXJ18);科技部农业科技成果转化资金项目(2009GB23260456)

摘　　要：目的:考察油菜蜂花粉核苷类成分的提取条件,确定最佳提取工艺。方法:先通过单因素试验获得油菜蜂花粉核苷类成分提取时间、提取温度及料液比的优化范围,再利用Box-Behnken试验设计原理和3因素3水平的响应面分析法优化蜂花粉核苷类成分的提取工艺。结果:3因素的优化范围分别为40～60min、45～65℃、1:60～1:80(g/mL)。结论:最终优化条件为提取时间53min、温度60℃、料液比1:70(g/mL),该条件下,油菜蜂花粉中核苷类成分总提取量为(705.2±0.006)mg/100g。Nucleosides extraction from rape bee pollen was optimized using response surface methodology. The appropriate ranges of extraction time, extraction temperature and material-to-liquid ratio were obtained by one-factor-at-a-time experiments for subsequent optimization using response surface analysis based on a three-variable, three-level central composite design. The optimal ranges of extraction time, extraction temperature and material-to-liquid ratio were 40-60 rain, 45 -65 ℃ and 1：60- 1：80 （g/mL）, respectively. The optimized extraction conditions were 53 min, 60 ℃, and 1：70 （g/mL）. Under these conditions, the extraction rate of nucleosides from rape bee pollen was （705.2 ± 0.006） mg/100 g.

关 键 词：响应面法 核苷类成分 油菜蜂花粉 Box-Behnken 超声波提取 优化 

分 类 号：S896.4[农业科学—特种经济动物饲养] TS201.2[农业科学—畜牧兽医]