两组天麻提取物的制备及对灰树花发酵的影响  

Preparation of Two groups of Gastrodia elata BL. Extracts and Their Effects on Grifola frondosa in Submerged Culture

作　　者：张勇[1] 徐晓宝[2] 王娜[1] 谭沙[1] 付红伟[3] 吴天祥[1] 

机构地区：[1]贵州大学生命科学学院,贵阳550025 [2]贵州大学化学与化工学院,贵阳550025 [3]浙江大学生物医学工程与仪器科学学院,杭州310027

出　　处：《食品工业》2012年第7期150-153,共4页The Food Industry

摘　　要：在灰树花深层发酵体系中,分别添加两组天麻的醇提液,分析其对灰树花菌体生长和胞外多糖(Extracellular Polysaccharide,EPS)合成的影响,利用HPLC测定两组天麻醇提液中天麻素的含量。结果显示,两组天麻醇提液的添加体积浓度在4%时均能显著促进灰树花菌体的生长和胞外多糖(EPS)的产量(p<0.05),然而试验组2的天麻醇提液对灰树花细胞生长和胞外多糖的促进作用优于试验组1。天麻素含量的测定显示,试验组2的天麻醇提液中的天麻素含量是试验组1的1.33倍。研究表明,不同加工条件的天麻,所制得的天麻醇提液对灰树花菌体生长和胞外多糖的影响效果差异显著,说明天麻制品的制作方法不同,将得到不同的使用效果。Two groups of alcohol extracts from different Gastrodia elata BL.s were separately added into the fermentation system of Grifola frondosa to analyze their effects on Grifola frondosa＇s mycelia gowth and extracellular polysaccharide（EPS） biosynthesis.And the gastrodia contents in different Gastrodia elata BL.s were determined by HPLC.The results showed that the 2 alcohol extracts from different Gastrodia elata BL.s in a medium containing 4%（V/V） both could promote biomass growth and extracellular polysaccharide（EPS） biosynthesis of Grifola frondosa significantly（p0.05）.However,the alcohol extract of Gastrodia elata BL.in the group 2 would more contribute to Grifola frondosa＇s biomass growth and EPS than that of the alcohol extracts Gastrodia elata BL.in the group 1.In addition,the results of gastrodia content showed that the gastrodia content in the alcohol extract of Gastrodia elata BL.in the group 2 was as 1.33 times as that of the alcohol extracts Gastrodia elata BL.in the group 1.So it was from Gastrodia etata BL.with different processing methods that there was a significant difference in effects of the alcohol extracts on mycelia growth and EPS of Grifola frondosa.

关 键 词：天麻 灰树花 天麻素 EPS 

分 类 号：TS201.2[轻工技术与工程—食品科学]