花生过敏原检测方法研究进展  

Research Progress in Peanut Allergen Detection

作　　者：韩远龙[1] 吴志华[1] 闫飞[1] 陈红兵[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,中德联合研究院,江西南昌330047

出　　处：《食品科学》2012年第13期305-308,共4页Food Science

基　　金：江西省科技支撑计划重点项目(2009BNA08100)

摘　　要：花生是重要食物过敏原之一,能引起严重的过敏反应。目前尚没有花生过敏的特效疗法,严格避免食入含花生的食物是花生过敏患者的最佳选择,所以食物中花生的检测就显得尤为重要。本文对花生主要过敏原及其过敏反应研究进展进行简要介绍,重点介绍花生过敏原的主要检测方法的新发展,包括传统的酶联免疫吸附实验(ELISA)、免疫印迹法、聚合酶链式反应(PCR)等,ELISA和免疫印迹法都是应用较为普遍的检验方法,PCR方法应用较少,但它能从DNA/RNA方面检测花生过敏原的存在;新兴检测方法主要包括生物传感器和质谱法,这两种方法在花生检测方面具有可观的前景。未来的这些检测方法将朝着快速、灵敏、简便的方向发展。Peanut is one of the major allergenic loods, which can cause severe allergic reactions. Currently, there are no effective therapeutic strategies for peanut allergy. For peanut allergic patients, avoiding intake of the foods containing peanuts is the best choice. Meanwhile, the detection of peanuts in foods is becoming particularly important. Herein, major peanut allergens and research progress in peanut allergen detection are introduced. Traditional enzyme-linked immunosorbent assay （ELISA）, west- ern blotting and polymerase chain reaction （PCR） are summarized. ELISA and western blotting are extensively used in the detection of peanut allergens. However, the application of PCR based on DNA or RNA is not common. Moreover, some newly developed detection methods such as biosensor and mass spectrometry are discussed. Both of them are characteristics of faster, easier and more sensitive detection have promising prospects in the detection of peanut allergens.

关 键 词：花生过敏 ELISA 免疫印迹法 PCR 生物传感器 质谱法 

分 类 号：TS207.3[轻工技术与工程—食品科学]