酸性木聚糖酶固态发酵条件与粗酶性质研究  

Solid-state fermentation conditions of acidic xylanase and its crude properties

作　　者：周晨妍[1] 刘振华[1] 郭伟云[1] 王燕[1] 李端[1] 

机构地区：[1]新乡医学院生命科学技术系,河南省医学遗传学与分子靶向药物高校重点实验室培育基地,河南新乡453003

出　　处：《中国酿造》2012年第7期65-68,共4页China Brewing

基　　金：河南省教育厅自然研究计划项目资助(No.2011A180026);河南省科技厅科技攻关项目(No.112102210299)

摘　　要：利用单因素和正交试验对实验室选育的黑曲霉XZ-3S（Aspergillus niger XZ-3S）菌株的发酵条件进行了研究。得到试验因素的产酶条件为天然原料麸皮与玉米芯的比例为5∶3,接种量1.0%（孢子悬液,孢子浓度5×107个/mL）,料水比例1∶1.7,培养基初始pH值为7.5,培养温度28℃、培养时间65h,其间翻曲2~3次,在上述条件下木聚糖酶的活力可达23612.38IU/g干曲。酶学性质初步研究显示,酶的最适反应温度和pH值分别为50℃和5.0,低于50℃、pH3.0~8.0酶稳定。The single-factor tests and orthogonal experiment were employed to evaluate the optimal fermentation conditions of xylanase produced by Aspergillus niger XZ-3S in this paper.The optimum fermentation conditions were as follows： the ratio of wheat bran：cob in medium 5∶3,the inoculum 1.0%,the ratio of solid to fluid 1∶1.7,the initial pH value 7.5,the temperature 28℃,and the time for fermentation 65h.Under those conditions,the enzyme activity of xylanase produced by the strain reached 23612.38IU/g dry medium.The optimal temperature and pH for xylanase for xylanase reaction were 50℃ and 5.0 respectively.The enzyme showed a high stability at pH value 3.0~8.0 below 50℃.

关 键 词：固态发酵 木聚糖酶 黑曲霉 正交试验 酶学性质 

分 类 号：TQ814[化学工程]