含绿原酸原料总黄酮检测方法的研究  

Study on the Identification of Flavonoids in Raw Materials with Chlorogenic Acid

作　　者：仇豪文[1] 沈晖[1] 曾里[1] 曾凡骏[1] 

机构地区：[1]四川大学轻纺与食品学院,四川成都省610065

出　　处：《食品与发酵科技》2012年第4期103-106,共4页Food and Fermentation Science & Technology

摘　　要：建立了一种适用于含绿原酸原料的总黄酮检测方法,选择杜仲叶作为原料,以ZrOCl2作为显色剂,以芦丁标品作为对照品,选用440nm作为测定波长,采用分光光度法检测总黄酮含量。结果表明:在0.0125-0.2mg范围内,芦丁浓度与吸光度呈良好线性关系,回归方程为y=3.3601x+0.0019,相关系数R2=0.9994,精密度实验RSD值为1.587%,样品平均加标回收率为101.63%。该方法操作简单,结果准确,适用于绿原酸含量较高的原料中总黄酮含量的检测。This research builted an improved method in identifying total flavonoids for raw materials which was rich in chlorogenic acid. Choosing the leaves of Eucommia ulmoides Oliver as the study object, the method required 2mL of 2% ZrOC12 solution as chromogenic reagent, lmL of acetic acid and sodium acetate buffer solution, a standing time of 70min and a scan wavelength of 440nm. The results showed that a good linear relationship （y=0.0336x＋ 0.0019 R2=0.9994） between OD and concentration of rutin within the range of 0-0.02mg. The average recovery rate was 98.59% with RSD 1.587%. This method is easy to operate and accurate.

关 键 词：总黄酮 绿原酸干扰 杜仲叶 检测 

分 类 号：TS207.3[轻工技术与工程—食品科学]