水溶性有机溶剂对鸡肝酯酶酶活力的影响  

Effects of Water-Soluble Organic Solvents on Activity of Chicken Liver Esterase

作　　者：赵洁[1] 徐斐[1] 曹慧[1] 于劲松[1] 

机构地区：[1]上海理工大学医疗器械与食品学院,上海200093

出　　处：《食品科学》2012年第15期216-219,共4页Food Science

基　　金：上海市科委生物医药专项(10391901500);上海市农委科技兴农重点攻关项目(2006第7-4号)

摘　　要：从新鲜鸡肝中提取出鸡肝酯酶,并研究水溶性有机溶剂对鸡肝酯酶酶活力的影响。结果表明:甲醇、乙醇、正丙醇、乙二醇-甲醚、乙二醇-乙醚在低体积分数时对鸡肝酯酶酶活力有激活作用,高体积分数使其失活。乙二醇-甲醚能够较好的保持鸡肝酯酶活力,当体积分数达到50%时,相对酶活力仍保持93%。丙酮和1,4-二氧六环对鸡肝酯酶主要表现为失活作用,当体积分数达到10%时,相对酶活力低于80%。Chicken liver esterase,isolated from fresh chicken liver,is available for detecting pesticides.The effects of watersoluble organic solvents on the activity of chicken liver esterase were investigated.The results indicated that methanol,alcohol,propanol,ethylene glycol monomethyl ether（EGME） and glycol ether at low concentration could activate chicken liver esterase;in contrast,these organic solvents at high concentration could inactivate chicken liver esterase.Ethylene glycol monomethyl ether was the best organic solovent to maintain the activity of chicken liver esterase.When EGME concentration was 50%,the relative activity of chicken liver esterase remained 93%.Acetone and dioxane had negative effect on enzymatic activity.Acetone and dioxane at a concentration of 10% could result in 20% loss for relative activity of chicken liver esterase.

关 键 词：鸡肝酯酶 酶活力 水溶性有机溶剂 

分 类 号：TS201.6[轻工技术与工程—食品科学] TS201.2[轻工技术与工程—食品科学与工程]