响应面法优化提取黑豆苗叶绿素工艺研究  

Study on Optimization Extraction Conditions of Chlorophyll from Black Bean Spout by Response Surface Methodology

作　　者：魏海香[1] 梁宝东[1] 张爱民[1] 王元军[1] 魏小叶[1] 

机构地区：[1]济宁学院生命科学与工程系,山东曲阜273155

出　　处：《北方园艺》2012年第18期11-14,共4页Northern Horticulture

基　　金：济宁市重大科技专项资助项目(2008GG201)

摘　　要：以黑豆芽苗为试材,在单因素试验基础上,根据BOX中心组合设计原理采用3因素5水平响应面分析法,对黑豆苗叶绿素的提取工艺进行了优化研究,并对各因素的显著性和交互作用进行了分析。结果表明:豆苗叶绿素的最佳提取工艺条件为:提取溶剂95%乙醇溶液,料液比1g∶4mL,浸提温度40℃,浸提时间9h;该条件下提取的叶绿素浓度可达到3.03mg/L。Abstract.Response surface methodology was used to optimize the extraction processing of chlorophyll from black bean sprout. On the basis of single factor experiments, three factors and five levers response surface methodology were designed by BOX centre modular design principle. The results showed that the optimal extraction conditions of chlorophyll were as follows ：extraction solvent 95 % alcohol,material-liquid ratio 1 g ＂ 4 mL,extraction temperature 40°C and extraction time 9 h. Under the optimal extraction conditions, the chlorophyll extraction concentration was up to 3. 03 mg/L.

关 键 词：豆苗叶绿素 提取 响应面法 工艺 

分 类 号：TS264.4[轻工技术与工程—发酵工程]