不同热作用条件下牛乳中5-HMF生成量的变化研究  

Study on the Quantities Change of the 5-Hydroxymethylfurfural in Milk under the Different Heat Treatments

作　　者：高萌[1] 葛武鹏[1] 张小军 崔璐璐[1] 秦立虎 

机构地区：[1]西北农林科技大学食品科学与工程学院,杨凌712100 [2]西安伊利泰普克饮品有限公司,西安710068 [3]西安奶业科学研究所,西安710065

出　　处：《食品工业》2012年第9期120-123,共4页The Food Industry

基　　金：西北农林科技大学人才基金资助项目Z111020923

摘　　要：旨在探讨5-羟甲基糠醛（5-HMF）作为牛乳中热作用程度标示物的可行性并指导不同热处理工艺参数确定的科学性。采用高效液相色谱（HPLC）法对不同温度和时间处理条件下牛乳中的5-HMF生成量变化进行分析比较，揭示其规律性变化。结果表明：（1）随着加热温度的升高和时间的延长，巴杀乳和超高温瞬时灭菌（UHT）乳中5-HMF的生成量均有相应变化，且加热温度越高，时间越长，5-HMF的生成量越高，表明5-HMF作为牛乳受热作用程度标示物可行。（2）可以快速测定牛乳中5-HMF生成量变化，结果具有较高准确度和重现性，5-HMF回收率为90．63％-96．23％。结论为：5-HMF作为标示物科学可行，基此确立的液态乳工艺参数为：巴杀乳温度控制在（85±2）℃，时间为15s；uHT乳温度控制在（137±2）℃，时间为4s。： To discuss the 5-HMF as the milk heat degree validation of target sensitivity and direct the scientific of determine the different heat treatment processes. A high performance liquid chromatographic （HPLC） method was developed for the quantities change of 5-Hydroxymethylfurfural （5-HMF） in milk under the different heating temperature and time, revealed its regularity changes. The results showed that： First, with increasing the heating temperature and heating time, the 5-HMF quantities in pasteurizing milk and instantaneous ultra high temperature sterilizing （UHT） milk all had changed. The higher the heating temperature and the longer the heating time the UHT was, the higher quantities of 5-HMF had. Second, Using this method could rapid determined the rule in quantities of 5-HMF and existed a well linear relationship between peak area and content, the determination results had high accuracy and reproducibility, and the recovery percent of 5-HMF was 90.63%-96.23%. The conclusion was that： 5-HMF as mark was scientific and feasible, based on this, establishment of the liquid milk for process parameters was： the pasteurization dairy temperature should be controlled at （ 85±2 ） ℃, the time for 15 s; the UHT dairy temperature should be controlled at （ 137±2 ） ℃, the time for 4 s.

关 键 词：5-羟甲基糠醛 生成量 加热温度 加热时间 

分 类 号：TS252.41[轻工技术与工程—农产品加工及贮藏工程]