微生物发酵法生产L-鸟氨酸的研究  

THE STUDIES ON FERMENTATION OF L-ORNITHINE

作　　者：陈宁[1] 潘韵[1] 方杰[1] 刘淑云[1] 张克旭[1] 

机构地区：[1]天津轻工业学院食品工程系,天津300222

出　　处：《天津轻工业学院学报》2000年第3期20-24,共5页Journal of Tianjin University of Light Industry

摘　　要：以谷氨酸棒杆菌为出发菌株 ,经硫酸二乙酯、紫外线诱变 ,定向选育出一株鸟氨酸生产菌A1 1 57,其遗传标记为 Arg- +D-Argr+SGr,并能耐受高浓度葡萄糖。对菌株 A1 1 57的发酵条件进行了研究 ,在最佳培养条件下 ,该菌株 L-鸟氨酸产量可达 9.85g/ L。Original strain, Corynebacterium glutamicum, was mutated by DES and U.V.Then a higher L-Ornithine producing strain——A1157 was obtained.It requires L-Arginine as essential growth factor.This mutant is resistant to D-Arginine and sulfaguanidine and could be cultured at high concentration glucose medium.The fermentation conditions of A1157 were studied.Under the optimum culture condition,the strain A1157 was able to accumulate an average of 9.85 g/L of L-Ornithine.

关 键 词：鸟氨酸 谷氨酸棒杆菌 育种 发酵 生产菌 诱变 

分 类 号：TQ922.9[轻工技术与工程—发酵工程]