响应面法优化无溶剂体系磷脂酶A1催化酯化制备低聚甘油酯的研究  

Optimization of preparation of polyglycerol fatty acid ester catalyzed by Phospholipase A1 in a solvent free system using response surface methodology

作　　者：肖伊莎[1] 汪勇[1] 张广文[1] 

机构地区：[1]暨南大学食品科学与工程系,广东广州510632

出　　处：《食品工业科技》2012年第20期191-194,198,共5页Science and Technology of Food Industry

基　　金：粤港关键领域重点突破项目(2009A020700003)

摘　　要：以工业油酸和低聚甘油为原料,经磷脂酶A1(Lecitase Ultra)催化酯化制备低聚脂肪酸甘油酯。利用响应面法优化实验条件,研究反应时间、反应温度、加酶量、加水量以及底物摩尔比值(工业油酸和低聚甘油摩尔比值)对其酯化率的影响。得出最佳反应条件为:反应温度45℃,加酶量1.6wt%(占底物总质量),加水量4wt%(占底物总质量),反应时间12h,底物摩尔比值为1:1。最佳条件下酯化反应平均酯化率可达到56.6%。采用HPLC-ESI质谱通过检测相对分子质量验证了部分低聚甘油酯产物的种类。Production of oligomerized polyglycerol fatty acid ester by esterification of industry grade oleic acid with oligomerized polyglycerol catalyzed by phospholipase Al （Lecitase Ultra） was investigated in this study. The reaction parameters including reaction time,reaction temperature,enzyme dosage,molar ratio of substrates （industry grade oleic acid to oligomerized polyglycerol） and water dosage were studied using response surface methodology（RSM）. Optimum conditions obtained by RSM were as follows：reaction temperature 45℃, enzyme dosage 1.6wt%（of the substrates mass） molar ratio of oliomerized plyglycerol to fatty acid 1：1, reaction time 12h and water dosage 4wt%. Under these conditions,esterification efficiency of the reaction mixture was 56.6%. Some oligomerized polyglycerol fatty acid ester molecular species were identified by HPLC-ESI-MS.

关 键 词：聚甘油酯 响应面法 磷脂酶A1 酯化 

分 类 号：Q559[生物学—生物化学]