活细胞传感仪在嗜酸乳酸杆菌培养过程中的应用  

The application of viable cell mass monitoring in Lactobacillus acidophilus cultivation

作　　者：罗艳霞[1] 黄明志[1] 郭元昕[1] 王泽建[1] 储炬[1] 

机构地区：[1]华东理工大学生物反应器国家重点实验室,国家生化工程技术研究中心,上海200237

出　　处：《食品工业科技》2012年第21期152-155,159,共5页Science and Technology of Food Industry

基　　金：十二五"科技支撑计划"(2011BAF02B03);国家高技术研究与发展计划(863计划)(2012AA021201);十二五"973"项目(2012CB721006)

摘　　要：活细胞是嗜酸乳酸杆菌发酵过程的目标产物,但长期以来一直缺乏一种能快速、准确地检测发酵液中活细胞量的方法,本文引入了一种基于电容原理的活细胞量测量新方法,探讨了其在嗜酸乳杆菌培养过程中在线应用的可行性。研究结果表明,活细胞传感仪测定的活细胞数与平板活菌计数法测定的菌体浓度有非常好的线性关系,相关系数达0.9944,在相同的实验条件下,仪器测定数据具有较好的重复性,基于电容值计算出的比生长速率比基于CER、OUR计算出的比生长速率更可信。因此活细胞传感仪为嗜酸乳杆菌培养过程在线监测提供了一种简便、精确的方法。Viable cell is the final products of Lactobacillus acidophilus fermentation process. However, at present, there is no efficient method to monitor it on-line quickly and accurately.In this paper,a new method that based on the capacitive principle for on-line monitoring viable-cell mass was employed.As a result,the relationship between the number of colony forming units（CFU） and capacitance with a correlation coefficient（R） of 0.9944 was achieved and data showed a good reproducibility under the same culture conditions.Simultaneously,compared with those determined by indirect online estimation methods including oxygen uptake rate （OUR）and carbon dioxide evolution rate （CER）, the specific growth rate estimated by on-line capacitance measurement could be more reliable during Lactobacillus acidophilus fermentation process .Therefore, it could be concluded that a capacitance probe was a simple as well as a accurate tool in Lactobacillus acidophilus cultivation process.

关 键 词：活细胞传感器 电容 嗜酸乳杆菌 

分 类 号：TS201.3[轻工技术与工程—食品科学]