泰和乌骨鸡活性肽对5-氟尿嘧啶诱导HSF细胞损伤的保护作用  

Protective effect of bioactive peptides from taihe black-bone silky fowls on human skin fibroblasts injured by 5-fluorouracil

作　　者：田颖刚[1] 王春艳[1] 黄宇玫[2] 廖春艳[1] 张盼[1] 朱胜[1] 乔娟娟[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047 [2]南昌大学科学技术学院,江西南昌330029

出　　处：《食品工业科技》2012年第21期356-360,共5页Science and Technology of Food Industry

基　　金：江西省青年科学家培养计划(2010)资助项目(赣科发计字[2010]209号)

摘　　要：目的:用单细胞凝胶电泳技术(SCGE)研究泰和乌骨鸡活性肽对5-氟尿嘧啶(5-FU)损伤细胞DNA的保护作用。方法:传代培养人皮肤成纤维细胞(HSF),随机分为正常对照组,5-FU组,5-FU+泰和乌骨鸡活性肽组。应用MTT法检测细胞存活率,单细胞凝胶电泳法(SCGE)检测5-FU引起HSF细胞DNA损伤程度,羟脯氨酸试剂盒检测细胞培养液中羟脯氨酸含量。结果:与正常对照组比较:5-FU组HSF细胞DNA的损伤程度较严重,且尾长、尾距、Olive尾距和尾部DNA百分含量显著增加(p<0.001),细胞培养液中羟脯氨酸含量显著性减少(p<0.05)。与5-FU组比较,泰和乌骨鸡活性肽组细胞的尾长、尾距、Olive尾距和尾部DNA百分含量均显著性减少(p<0.001),而细胞培养液上清中羟脯氨酸含量升高(p<0.01)。结论:泰和乌骨鸡活性肽对HSF细胞DNA损伤具有保护作用,泰和乌骨鸡活性肽可以剂量依赖性地降低5-FU诱导的HSF细胞DNA的断裂损伤。Objective ： A DNA injury model was established by using 5- fluorouracil （ 5- FU ） in this study. Methods ： A series of experiments （ MTT assay, single cell gel electrophoresis （ SCGE）, and hydroxyproline assay kits） were designed to investigate the effect of bioactive peptides from Taihe Black-Bone Silky FowI（TBSF）on Human Skin Fibroblasts （HSF） against 5- FU- induced DNA damage.Cultured HSF were randomly divided into three groups ： the control group,5-fluorouracil group and 5-fluorouracil ＋ bioactive peptides from TBSF.Cell viability was measured by MTT assay, DNA damage was detected by SCGE assay, and hydroxyproline levels in HSF culture medium were measured by hydroxyproline assay kits. Results： compared with the control group,5-FU group exacerbated the DNA damage,in which the tail length,the tail moment,the Olive tail moment,and the tail DNA content significantly increased （ p 〈 0.001 ）, whereas the content of hydroxyproline in HSF culture medium significantly decreased （ p 〈 0. 05）.Compared with 5-FU group, Bioactive peptides from TBSF attenuated 5-FU-induced DNA damage, in which the tail length,the tail moment,the Olive tail moment and the tail DNA content significantly decreased （p 〈 0.05）, whereas the content of hydroxyproline increased dramatically（ p 〈 0.01 ）.Conclusion： Bioactive peptides from TBSF can protect HSF against 5-FU-induced DNA damage in a dose-dependent manner.

关 键 词：泰和乌骨鸡 活性肽 5-氟尿嘧啶 DNA损伤 单细胞凝胶电泳 

分 类 号：TS201.4[轻工技术与工程—食品科学]