从长春地区牛肉和猪肉中检出产vero毒素大肠杆菌O157∶H7  

Isolation of Verotoxin producing Escherichia coli O157∶H7 from Beef and Pork in Changchun

作　　者：周志江[1] 黄上缓 郑明光 郑昊 孙伟斌 张让堂 汪力亚[2] 王丽 李景云[2] 

机构地区：[1]解放军军需大学兽医学院,吉林长春130062 [2]解放军302医院,北京100039 [3].解放军军需大学兽医学院,吉林长春130062

出　　处：《中国兽医学报》2000年第2期152-155,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目

摘　　要：用分离培养法、生化反应鉴定、胶乳凝集试验和聚合酶链式反应 ( PCR)对长春地区市售的 4 0份牛肉和 3 0份猪肉样品进行了调查 ,结果从 2份牛肉 ( 5%)和 1份猪肉 ( 3 .3 %)样品中检出了产 vero毒素大肠杆菌 O1 57∶A tolal of 70 samples of retail beef and pork from Changchun, China were assayed for the presence of Escherichia coli serogroup O157∶H7 by a multiple procedure. The procedure involves several steps, including selective enrichment, sorbitol MacConkey agar (SMAC) culture, biochemical profile, slide and tube agglutination test and polymerase chain reaction (PCR). E.coli O157∶H7 was isolated from 2(5%) of 40 beef and 1 (3.3%) of 30 pork samples.

关 键 词：vero毒素 大肠杆菌 牛肉 猪肉 O157:H7 PCR 

分 类 号：R155.55[医药卫生—营养与食品卫生学] S852.612[医药卫生—公共卫生与预防医学]