碱性过氧化氢法提取玉米皮戊聚糖及其氧化交联性质的研究  

Study on Pentosan from Corn Fiber by Alkaline Peroxide Extraction and Oxidative Cross-linking Property of Pentosan

作　　者：王文侠[1] 张晓静[1] 宋春丽[1] 李建强[1] 

机构地区：[1]齐齐哈尔大学食品与生物工程学院齐齐哈尔大学农产品加工黑龙江省普通高校重点实验室,黑龙江齐齐哈尔161006

出　　处：《中国食品学报》2012年第10期96-101,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：黑龙江省科技厅攻关研究资助项目(GC10B402)

摘　　要：以玉米皮为原料,对碱性过氧化氢提取玉米皮中的戊聚糖提取条件以及戊聚糖的氧化交联性质进行研究。在考察H2O2溶液的体积分数、H2O2的pH值、固液比、提取时间、提取温度5个因素对戊聚糖得率影响的基础上,根据Box-Benhnken中心组合试验设计原理,采用四因素三水平响应面分析法,确定戊聚糖提取的最优条件是:固液比为1∶25(m/V),H2O2溶液体积分数2.14%,pH11.65,提取温度85℃,提取时间2.23h,此条件下戊聚糖得率达到47.18%。戊聚糖的氧化交联性与氧化剂的用量有关,用量过多或过少均会使氧化交联程度减弱。在糖质量分数为1%时,(NH4)2S2O8、KBrO3及过氧化氢与过氧化物酶(H2O2/POX)3个体系中氧化剂的最适用量分别为0.75%,0.17%,10U/g戊聚糖,此时有利于戊聚糖氧化凝胶的形成。Extracting pentosan was studied by alkaline peroxide treatment of the corn fiber. Extracted pentosan with ammomium persulfae, potassium bromate and hydrogen peroxide/peroxidase was studied. The effects of the concentration of hydrogen peroxide, pH, ratio of solid to liquid, temperature, extraction time and temperature on the yield of pentosan were studied respectively. Based on the results, other four key factors except ratio of solid to liquid were optimized through response surface methodology with four factors and three levels based on the principle of Box-Behnken design. The results showed that the optimum extraction conditions were 85 ℃, hydrogen peroxide concentration 2.14%, pH 11.65, 2.23 h, ratio of substrate and water 1∶25（m/V）. Under this optimal conditions, the yield of pentosan was highly reach to 47.18%. Extracted pentosan with ammomium persulfae, potassium bromate and hydrogen peroxide/peroxidase gave a slow increase in viscosity and when pentosan concentration was 1%（m/V）, optimum concentration of three oxidant was 0.75%, 0.17%, 10 U/g pentosan respectively.

关 键 词：玉米皮 戊聚糖 过氧化氢 碱提 响应面分析 氧化交联性 

分 类 号：TQ914.1[化学工程]