赤藓糖醇高产菌选育及发酵条件研究  

Study on breeding of high erythritol producing strain and fermentation condition

作　　者：温拥军[1,2] 游玟娟[1] 罗跃中[1] 谢达平[2] 

机构地区：[1]湖南化工职业技术学院,湖南株洲412004 [2]湖南农业大学生物科学技术学院,湖南长沙410128

出　　处：《食品工业科技》2012年第22期242-245,共4页Science and Technology of Food Industry

摘　　要：为了提高赤藓糖醇的产量,对自选耐高渗酵母菌T-3-2进行紫外线与亚硝酸复合诱变处理,并采用响应曲面法优化了其发酵条件。复合诱变得到1株稳定高产突变株UN-11,其赤藓糖醇的产量达到78.3mg/mL,比T-3-2提高了38.8%;通过响应面分析建立了关键影响赤藓糖醇产量的二次多项式数学模型,得到最佳生产工艺条件为:发酵温度31℃、转速170r/min、接种量9.8%。模型预测结果产物浓度达到84.93mg/mL,验证实验结果产物浓度为85.25mg/mL。该模型对赤藓糖醇工业化生产有一定的指导意义。In order to improve the yield of erythritol,the original strain osmotolerant yeast T-3-2 was treated by UV and HNO2,and its fermentation condition was optimized by single factor and response surface methodology（RSM）.A high and stable yield mutant named UN-11 was obtained.The erythritol yield of UN-11 was 78.3mg/mL,which increased 38.8% compared with the original strain.The second-order polynomial equation for erythritol yield was established by response surface analysis theory.Optimized processing conditions obtained were fermentation temperature of 31℃,rotate speed of 170r/min and inoculation amount of 9.8%（v/v）.Under these optimized conditions,the predicted yield of fitted model was 84.93mg/mL and verifying test result was 85.25mg/mL.The model had some guiding sense for erythritol industrial production.

关 键 词：赤藓糖醇 耐高渗酵母 紫外线 复合诱变 响应曲面法 

分 类 号：TS201.3[轻工技术与工程—食品科学]