响应面法优化超声波提取川皮苷工艺研究  

Optimization of Ultrasonic-Assisted Nobiletin Extraction by Response Surface Methodology

作　　者：张玉[1,2] 李洪军[1] 窦华亭[2] 吴厚玖[2] 贺稚非[1] 王华[2] 孙志高[2] 马亚琴[2] 

机构地区：[1]西南大学食品科学学院,重庆400715 [2]中国农业科学院(西南大学)柑桔研究所,重庆400712

出　　处：《食品科学》2012年第20期123-127,共5页Food Science

基　　金：西南大学优博论文培育项目(Kb2010020)

摘　　要：为确定甜橙皮渣中川皮苷超声提取的最佳工艺,以甜橙皮渣为原料,在单因素试验基础上采用Box-Behnken试验设计和响应面分析方法,建立超声功率、超声时间、超声温度和料液比与川皮苷得率之间的数学模型。结果表明:回归方程显著,决定系数R2=0.9319,一次项和二次项对川皮苷得率有显著影响。超声波提取川皮苷的最佳工艺条件为料液比1:20(g/mL)、超声温度59.0℃、超声时间45min、超声功率120W。此工艺条件下,川皮苷得率为209μg/g,与模型预测值204μg/g相近。The purpose of this study is to optimize operating conditions for ultrasonic-assisted extraction of nobiletin from sweet orange residues by response surface methodology.One-factor-at-a-time designs were used to examine the effects of four extraction parameters including ultrasonic power,ultrasonic treatment time,temperature and solid-to-solvent ratio on nobiletin yield.Further a mathematical model for nobiletin yield as a function of the extraction parameters was constructed by regression analysis based on a Box-Behnken design.The mathematical model was statistically significant and its determination coefficient（R2） was 0.9319.Moreover,the linear and quadratic terms had significant effects on nobiletin yield.Optimal extraction conditions were obtained by the use of absolute ethanol at a solid-to-solvent ratio of 1：20 g/mL and ultrasonic treatment for 45 min at 120 W.Under the optimal conditions,the experimental nobiletin yield was 209 μg/g and in good agreement with the predicted value（204 μg/g）.

关 键 词：甜橙皮渣 川皮苷 超声波 响应面分析 

分 类 号：TS209[轻工技术与工程—食品科学]