大豆乳清中β-淀粉酶的超滤提取技术研究  

Extraction of β-amylase from the soybean whey wastewater by ultrafiltration technology

作　　者：陈超琴[1] 赵黎明[1] 蒋丽华[1] 夏泉鸣[1] 

机构地区：[1]华东理工大学发酵工业分离提取技术研发中心,华东理工大学食品科学与工程系,上海200237

出　　处：《食品工业科技》2012年第23期240-243,共4页Science and Technology of Food Industry

摘　　要：大豆乳清废水中含有丰富的β-淀粉酶。通过超滤技术对乳清废水中的β-淀粉酶进行浓缩回收,研究了膜截留分子量(MWCO)、操作温度、料液pH对超滤效果的影响。结果表明,选用MWCO3ku的膜,在跨膜压差2bar、温度(25±3)℃、pH7的操作条件下,膜通量由初始的44L/(m2·h)衰减至23L/(m2·h),平均通量达到35L/(m2·h);β-淀粉酶的酶活力可达到101U/mL,料液的浓缩倍数为10倍,酶活浓缩倍数约为9倍,总酶活的回收率为92%,浓缩液较高的β-淀粉酶酶活力为下一阶段的分离纯化奠定了基础,为大豆深加工废水综合利用提供了依据。Ultrafiltration technology was used to concentrate the β-amylase from the soybean whey wastewater which displays high β-amylase activity. The optimization of the UF process which involved the selection of membrane molecular weight cut off （ MWCO）, operating temperature and the pH of the feed was studied. Experiment results showed that polyethersulfone membrane with molecular weight cut off（MWCO）3ku could be applied to enrich the β- amylase signally at trans- membrane pressure （TMP） ： 2bar; pHT.0,25 ± 3℃, the flux decreased from 44L/（m^2 .h） to 23L/（ m^2 · h）, got an average flux of 35L/（ m^2 · h） .The β- amylase in the retentate got an activity of 101U/mL with 92% yield, with a volumetric concentration ratio of ]0 times, and the concentration ratio of enzyme activity was about 9 times.β-amylase in the retentate with a relatively high activity, which provided a good foundation for the further purification of the β-amylase.Meanwhile,this technology provided a new thought to make good use of the soybean whey wastewater for the Chinese manufacturers.

关 键 词：Β-淀粉酶 大豆乳清废水 超滤 

分 类 号：TS214[轻工技术与工程—粮食、油脂及植物蛋白工程]