α-环糊精葡萄糖基转移酶菌株的筛选与鉴定  

Selecting and identification of the producing strain for α-Cyclodextrin Glycosyltransferase

作　　者：张智维[1] 雷新辉[1] 张海群[1] 

机构地区：[1]陕西科技大学生命科学与工程学院,陕西西安710021

出　　处：《陕西科技大学学报（自然科学版）》2012年第6期89-92,共4页Journal of Shaanxi University of Science & Technology

基　　金：陕西省自然基金项目(2009JM2005);陕西省教育厅专项科研计划项目(09JK372)

摘　　要：从富含淀粉的土样中,初筛出22株产α-环糊精葡萄糖基转移酶菌株,然后用透明圈法筛选出3株酶活力较高的菌株,最后绘制酶活力曲线,得到一株酶活力为0.64U/mL的菌株N1.通过菌落形态、显微观察和生理生化特性,鉴定N1为肠杆菌科的产酸克雷伯氏菌.22 strains of bacteria which could yield α-CGTase were prescreened from soil sire ples. These soils are rich in starch. 3 strains of them with higher enzymatic activity were further selected by assaying transparent zone. Finally, we obtained a strain with activity of 0.86 U/mL from setting up a standard curve of enzymatic activity. This research showed that N1 was a Klebsiella oxytoca based on its colony morphology, microscopic observation and physiology and biochemical characteristic.

关 键 词：Α-环糊精葡萄糖基转移酶 筛选 鉴定 酶活力 

分 类 号：Q555.4[生物学—生物化学]