纤维素酶对油菜蜂花粉的破壁作用  

Cell Wall Disruption of Rape Bee Pollen by Cellulase

作　　者：孟良玉[1] 蔡文倩[1] 兰桃芳[1] 卢佳琨[1] 周璐[1] 励建荣[1] 

机构地区：[1]渤海大学化学化工与食品安全学院,辽宁省食品质量安全重点实验室,辽宁锦州121013

出　　处：《食品科学》2012年第22期72-75,共4页Food Science

基　　金：辽宁省食品安全重点实验室开放课题;渤大博士启动科研基金项目(BSQD2010-006)

摘　　要：研究纤维素酶对油菜蜂花粉破壁的影响。通过单因素试验分别考察料液比、加酶量、酶解温度、酶解时间对油菜蜂花粉破壁的影响,以油菜蜂花粉破壁率和多糖提取率为指标,根据单因素试验结果设计正交试验,得出油菜蜂花粉的最佳破壁条件为料液比1:50(g/mL)、加酶量为1.75%、酶解温度47.5℃、酶解时间5.0h,此条件下的油菜蜂花粉的破壁率为85.72%,多糖提取率为18.85%。The effects of solid-to-liquid ratio,enzyme loading,temperature and hydrolysis time on the efficacy of cellulase for cell wall disruption of rape bee pollen were examined in this study.One-factor-at-a-time and orthogonal array design methods were used to optimize the experimental conditions based on cell wall disruption rate and polysaccharide yield.The optimal conditions for cell wall disruption of rape bee pollen were found to be hydrolysis at 47.5 ℃ for 5.0 h with a solid-to-liquid ratio of 1：50（g/mL） and an enzyme loading of 1.75%,resulting in a cell wall disruption rate of 85.72% and a polysaccharide yield of 18.85%.

关 键 词：油菜蜂花粉 纤维素酶 破壁 显微镜观察 多糖 

分 类 号：S896.4[农业科学—特种经济动物饲养]