微波消解-氢化物发生原子荧光法同时测定紫薯中的硒和锗  

Simultaneous determination of selenium and germanium in purple sweet potato by microwave digestion-hydride generation atomic fluorescence spectrometry

作　　者：杨潇[1] 芮光伟[1] 徐和[1] 

机构地区：[1]西华大学生物工程学院,成都610039

出　　处：《食品科技》2012年第12期291-294,共4页Food Science and Technology

基　　金：西华大学食品生物技术省级重点实验室开放研究基金项目(SZJJ2009-005)

摘　　要：目的:建立一种微波消解-氢化物发生原子荧光法同时测定紫薯中硒和锗含量的方法。方法:以微波消解为前处理,在优化仪器分析条件和反应试剂的基础上,采用氢化物发生原子荧光法同时测定紫薯中的硒和锗。结果:此方法硒元素标准曲线的回归方程为:Y=61.998X+5.130,R=0.9997,线形范围为0.5～10ng/mL,检出限为0.19ng/mL;锗元素标准曲线的回归方程为:Y=153.69X-106.4,R=0.9934,线形范围为0.5～10ng/mL,检出限为0.40ng/mL。精密度RSD均小于2%,重现性RSD均小于5%。样品的加标回收率在79.96%～102.22%。结论:法简便,准确,灵敏度高,重现性好,可用于同时检测紫薯中硒和锗的含量。Objective： Experiment with microwave digestion-hydride generation atomic fluorescence spectrometry simultaneous determination of selenium and germanium in purple sweet potato. Method： The use of microwave digestion for the pre-treatment and the use of atomic fluorescence spectrometry for simultaneous determination of selenium and germanium in purple sweet potato based on optimization of the instrumental parameters, chemical parameters. Results： The selenium content of the regression equation： Y=61.998X＋5.130, R=0.9997, the linear range of 0.5-10 ng/mL, Minimum detectable value of selenium was 0.19 ng/mL. The germanium content of the regression equation： Y=153.69X-106.4, R=0.9934, the linear range of 0.5-10 ng/mL, Minimum detectable value of selenium was 0.40 ng/mL. RSD of precision was less than 2%, RSD of reproducibility was less than 5%, sample recoveries was 79.96%-102.22%. Conclusion： This method was simple, accurate, sensitive and reproducible, and can be used for simultaneous determination of selenium and germanium in purple sweet potato.

关 键 词：硒 锗 氢化物发生 原子荧光 微波消解 紫薯 

分 类 号：TS207.3[轻工技术与工程—食品科学]