麦曲中高淀粉糖化酶生产菌的分离鉴定及酶学特性研究  

Isolation and identification of glucoamylase producing strain from wheat Qu and research on enzyme properties

作　　者：于丽娟[1] 丁斐[1] 叶辉[1] 

机构地区：[1]南通大学生命科学学院,江苏南通226019

出　　处：《中国酿造》2012年第12期57-59,共3页China Brewing

基　　金：江苏省科技计划项目(SBK201240642);南通市科技计划项目(BK2011007);南通大学引进人才基金项目(03080477)

摘　　要：该实验利用透明圈平板初筛，固态发酵测定酶活复筛的方法从黄酒麦曲中筛选到一株产淀粉糖化酶活力较高的菌株A17，其初始酶活为1835U／g干曲。根据其形态特征和ITS序列分析，该菌株为米曲霉（Aspergillusoryzae）。该糖化酶的最适温度为60℃，在30℃～55℃内酶比较稳定，最适作用pH值为4．0；Ca2＋、Mg2＋对酶有一定的激活作用，而Fe3＋、Mn2＋、Cu2＋有较强的抑制作用。A starch-digesting glucoamylase producing strain A17 was isolated from wheat Qu by primary screening with transparent circle medium and rescreening with solid-state fermentation. The glucoamylase activity was 1835U/g medium（dry basis）. Based on its morphological characteristics and ITS sequence analysis, the strain was identified as Aspergillus oryzae. The optimum conditions were as follows, temperature 60℃, pH 4.0. The enzyme was stable at 30℃-55℃, and can be activated by Ca2＋ and Mg2＋, while inhibited by Fe3＋, Mn2＋ and Cu2＋.

关 键 词：淀粉糖化酶 固态发酵 酶学性质 

分 类 号：TS261.1[轻工技术与工程—发酵工程]