海蓬子籽油亚油酸共轭化产物的抗肿瘤作用  

Antitumor Activity of Conjugated Linoleic Acid Converted from Linoleic Acid in Salicornia herbacea Seed Oil

作　　者：吴演[1] 杨瑞琦[1] 陈美珍[1] 廖绪标[1] 

机构地区：[1]汕头大学理学院生物系,广东汕头515063

出　　处：《食品科学》2012年第23期318-322,共5页Food Science

基　　金：广东省教育部省部产学研合作专项资金项目(2010B090400461);广东省自然科学基金研究团队项目(S2011030005257)

摘　　要：目的:探讨海蓬子籽油亚油酸共轭化产物体外抗肿瘤活性。方法:以含有73.52%亚油酸的海蓬子籽油为原料,利用化学法转化制备共轭亚油酸(conjugated linoleic acid,CLA),采用国标法和小鼠急性毒性实验对共轭化油进行理化指标分析和急性毒性评价,MTT法检测共轭化油对人肿瘤细胞的生长抑制作用。结果:共轭化油中c9,t11-CLA和t10,c12-CLA含量为68.75%,理化指标符合相关产品要求。小鼠急性毒性LD50>20g/kg,属无毒级。共轭化油对食道癌EC109细胞和乳腺癌HTB-22细胞均有较强抑制作用,对宫颈癌HeLa细胞无抑制活性,对正常人胚肾细胞293T无毒性,对EC109细胞的IC50为111.9μg/mL。结论:利用海蓬子籽油转化的共轭亚油酸产物对人肿瘤细胞有较强的生长抑制作用,但其作用具有选择性。Objective： To explore the in vitro antitumor activity of conjugated linoleic acid（CLA） converted from linoleic acid in Salicornia herbacea seed oil.Methods： CLA was chemically prepared from Salicornia herbacea seed oil containing 73.52% linoleic acid and analyzed for physiochemical properties and acute toxicity in mice.Meanwhile,its growth inhibitory effect on human tumor cells was measured by MTT assay.Results： The total content of c9,t11-CLA and t10,c12-CLA in the prepared CLA was 68.75%.Its physiochemical properties met relevant quality requirements.Acute toxicity test showed an LD50 larger than 20 g/kg,indicating its nontoxicity.Esophageal cancer cells EC109 and breast cancer cells HTB-22 were powerfully inhibited by this CLA with an IC50 of 111.9 μg/mL for the latter,but It had no inhibitory effect on cervical cancer cells HeLa cell and no toxicity to normal embryos renal cell 293T.Conclusion： The CLA prepared from Salicornia herbacea seed oil has a strong growth inhibition on some human tumor cells,but this inhibition is cell type specific.

关 键 词：海蓬子籽油 共轭亚油酸 抗肿瘤作用 

分 类 号：Q939.139[生物学—微生物学]