硅胶载体氯霉素免疫亲和柱的制备  

Preparation of Silica Gel Immunoaffinity Column for Chloramphenicol Determination

作　　者：张灿[1,2] 周婷[1] 陆介宇[1] 段玉清[1] 王硕[2] 于娇[2] 

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013 [2]天津科技大学食品工程与生物技术学院,天津300457

出　　处：《食品科学》2012年第24期352-355,共4页Food Science

基　　金：国家自然科学基金青年科学基金项目(31000783);江苏大学优秀人才基金项目(09JDG052);江苏大学第11批大学生科研立项项目(11A349)

摘　　要：以硅胶为载体,通过硅烷偶联剂3-氨基丙基三乙氧基硅烷在其表面引入氨基,采用戊二醛法与氯霉素抗体进行偶联。该方法制备的免疫亲和柱对氯霉素抗体的偶联率达到72%,对氯霉素的最大吸附容量达到80ng/mL柱体积,选择含70%甲醇的PBS为洗脱溶液。以牛奶为检测对象分别采用3种不同方法进行样品处理,利用免疫亲和柱实现对样品中痕量残留的氯霉素纯化富集并进行ELISA检测。Silica gel was used as a carrier for preparing an immunoaffinity column （IAC） to determine chloramphenicol. Amino groups were introduced to the surface of silica gel using 3-aminopropyltriethoxysilane （APTES） as a silane coupling agent and the modified silica gel was conjugated with anti-chloramphenicol antibodies using glutaraldehyde. As a result, a conjugation ratio of 72% was obtained. The maximum adsorption capacity of the prepared IAC was 80 ng/mL. The elution solvent was PBS containing 70% methanol. After sample pretreatment by three different methods, chloramphenicol residues in milk were ourified and enriched using the IAC and determined by ELISA

关 键 词：免疫亲和柱 硅胶 氯霉素抗体 戊二醛法 

分 类 号：TS201.6[轻工技术与工程—食品科学] R978.13[轻工技术与工程—食品科学与工程]