1株产细菌素海洋乳酸菌的分离筛选及鉴定  

Screening and Identification of a Bacteriocin-producing Marine Lactic Acid Bacterium

作　　者：赵鸭美[1] 刘林[2] 安静莹[1] 胡雪琼[1] 刘颖[1] 

机构地区：[1]广东省水产品加工与安全重点实验室广东普通高等学校水产品深加工重点实验室广东海洋大学食品科技学院,广东湛江524088 [2]贵州大学生命科学学院,贵州贵阳550025

出　　处：《热带作物学报》2013年第1期171-175,共5页Chinese Journal of Tropical Crops

基　　金：广东省科学技术厅项目"海洋微生物源天然食品防腐剂的筛选与应用"(No.2010B020313002)

摘　　要：采用牛津杯打孔法,以大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌、单核细胞增生李斯特菌、副溶血弧菌、米曲霉为指示菌,对从南海海域中捕捞的华贵栉孔扇贝肠道中分离到的9株乳酸菌进行抗菌活性测定,并对活性菌株的发酵液进行排酸、排过氧化氢,以及胰蛋白酶、木瓜蛋白酶、胃蛋白酶、蛋白酶K酶解实验,筛选出1株具有较强抑菌作用产细菌素的乳酸菌ZH-54。结合形态鉴定、生理生化特性实验以及16S rRNA序列同源性分析,鉴定菌株ZH-54为坚强肠球菌(Enterococcus durans),其16S rRNA在GeneBank的登录号为JX258806。In this research, nine lactic acid bacteria strains were isolated from intestinal tract of a Chlamys nobilis from South China Sea. Their antibacterial activity to Escherichia coli, Staphylococcus aureus, bacillus subtilis, Listeria monocytogenes, vibrio prholyticus and Aspergillus oryzac were detected by Oxford plate double- layer agar experiment, and the antibacterial active strains were further studied to exclude the inhibitive effect of organic acid, H2Oz, and to observe the sensitivity of four proteases＇. The research found that lactic acid bacterium ZH-54 was a bacteriocin-producing strain. ZH-54 was identified as Enterococcus durarts on the basis of morphological and physiological-biochemical characteristics and phylogenetic tree, and the accession number of 16s rRNA in GenBank was JX258806.

关 键 词：乳酸菌 细菌素 抑菌试验 16S RRNA 

分 类 号：Q939.117[生物学—微生物学]