耐高渗糖蜜酿酒酵母的紫外诱变逐级驯化选育  

Breeding of Osmotolerant Molasses Saccharomyces cerevisiae with UV Mutagenesis and Gradual Demostication

作　　者：徐日益[1] 梁磊[1] 郭艺山[1] 高亚飞[2] 高俊永[1] 尚红岩[1] 黄向阳[1] 

机构地区：[1]广州甘蔗糖业研究所广东省甘蔗改良与生物炼制重点实验室,广东广州510316 [2]广西大学轻工与食品工程学院,广西南宁530004

出　　处：《甘蔗糖业》2012年第6期39-42,共4页Sugarcane and Canesugar

基　　金：广东省自然科学基金;项目编号10451031601006220

摘　　要：以酿酒酵母AS2.1189为出发菌,经过紫外诱变驯化100代,筛选得到B2和A17驯化株。将其接种于34゜Bx糖蜜发酵培养基,结果 A17发酵速率提升约12%,B2发酵速率提升约6%,表明驯化株耐高渗性能有较大提高。AS2.1189, the parent strain, was mutagenized by UV radiation and domesticated for 100 generations. B2 and A17 were screened, which were inoculated to 34°Bx molasses fermentation medium. Results show that the fermentation velocity of B2 and A17 was increased by 6% and 12%. The study indicates that the 2 mutants are more osmo-tolerant than the parent.

关 键 词：紫外诱变 驯化 糖蜜 酿酒酵母 耐高渗 

分 类 号：TS249.3[轻工技术与工程—制糖工程]