不同处理条件小黑豆乳清蛋白的抗肿瘤作用  

Anti-Tumor Activity of Whey Protein from Small Black Soybean Subjected to Treatment at Different Temperatures

作　　者：王菲[1] 王常青[1] 樊迎[1] 于书佳[1] 原敏[1] 连伟帅[1] 

机构地区：[1]山西大学生命科学学院,山西太原030006

出　　处：《食品科学》2013年第3期267-270,共4页Food Science

基　　金：山西省科技攻关计划项目(20100321102)

摘　　要：目的:研究不同温度处理的小黑豆乳清蛋白对S180动物模型的影响。方法:将昆明小鼠腋下接种S180腹水瘤后随机分组,65℃和75℃蛋白高、低剂量组分别按10g/(kg.d)和3g/(kg.d)喂食,以腹腔注射环磷酰胺为阳性对照组,研究小黑豆乳清蛋白对荷瘤小鼠的抑瘤率、脏器指数及免疫功能的影响。结果:经75℃处理的小黑豆乳清蛋白在3g/(kg.d)和10g/(kg.d)摄入剂量时,没有致胰腺肿大作用,对S180肿瘤的抑制效果优于65℃处理组,且瘤与体质量比显著低于65℃处理组(P<0.05);75℃乳清蛋白组的胸腺指数较其他各组明显提高(P<0.05),与基础组处于同一水平;与模型组相比,该组动物的白细胞数、肿胀度和半数溶血值极显著升高(P<0.01),且显著高于65℃处理组(P<0.05)。结论:小黑豆乳清蛋白经过75℃处理后能显著提高抗S180肿瘤的能力。Objective： To explore the effect of small black soybehn whey protein subjected to treatment at different temperatures on S180 mouse model. Methods： The S180-bearing mice were administered with whey proteins subjected to treatments at 75 and 65 ℃ at the doses of 10 g/（kg d） and 3 g/（kg d）, respectively. The mice administered with cyclophosphamide as the positive control. The inhibitory rate of tumors, organ index and immunologic function were measured. Results： The whey protein subjected to treatment at 75 ℃ at the doses of 3 g/（kg d） and 10 g/（kg d） did not result in the swelling of pancreas of the mice, and revealed more obvious inhibitory effect on S180 tumor than the whey protein subjected to treatment at 65 ℃ （P 〈 0.05）. The thymus index of mice administered with 75 ℃ whey protein remained at the basal level, which was significant higher than that of other groups （P 〈 0.05）. In addition, white blood cells, ear-swelling degree and HC50 of mice from 75℃ whey protein group were significantly higher than those of mice from the model group （P 〈 0.01） and 65℃ whey protein group （P 〈 0.01）. Conclusion： The whey protein from the small black soybean subjected to treatment at 75℃has obvious anti-tumor capability on S180 tumors.

关 键 词：小黑豆乳清蛋白 处理温度 抗肿瘤 

分 类 号：TS201.4[轻工技术与工程—食品科学]