儿茶素和咖啡碱组合对3T3-L1细胞增殖及脂肪代谢的影响  

THE COMBINED EFFECTS OF CATECHINS AND CAFFEINE ON CELLULAR PROLIFERATION AND LIPID METABOLISM IN 3T3-L1 CELLS

作　　者：郑国栋[1] 邱阳阳[1] 张清峰[1] 徐峰[1] 

机构地区：[1]江西省功能食品研究与开发重点实验室,江西农业大学食品科学与工程学院,南昌330045

出　　处：《营养学报》2013年第1期60-63,共4页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.31160320);江西省教育厅科技项目(No.GJJ10425)

摘　　要：目的研究对儿茶素和咖啡碱对3T3-L1细胞的增殖及脂肪代谢的影响。方法采用四甲基偶氮唑盐比色法(MTT)检测对3T3-L1细胞增殖的影响;3T3-L1细胞诱导分化8d后,对各组细胞进行油红O染色并测定细胞内甘油三酯(TG)含量;细胞分化12d后,添加儿茶素和咖啡碱组合或同时添加去甲肾上腺素(NA)作用24h,分析各组细胞内脂肪分解。结果儿茶素能明显抑制3T3-L1细胞的增殖;儿茶素和咖啡碱组合能明显抑制3T3-L1细胞分化后,细胞内TG的沉积,且在相同儿茶素浓度下,咖啡碱浓度越高抑制效果越明显。咖啡碱明显提高NA诱导成熟脂肪细胞脂解的能力,且呈剂量效应关系。结论儿茶素和咖啡碱组合能够抑制脂肪细胞增殖和甘油三酯积聚,咖啡碱促进激素诱导脂肪细胞中脂肪分解。Objective To investigate the combined effects of catechins and caffeine on cells proliferation and hpld metabolism in 3T3-L1 cells. Method MTT colorimetry was used to detect the effects of catechins and caffeine combination on the proliferation of 3T3-L1 cells. The differentiation of 3T3-L1 cells was induced for 8 d, then the adipocytes were stained by oil Red O, and the level of triglyceride （TG） was measured. The lipolytic effect of catechins and caffeine combination in presence or absence of noradrenaline （NA） for 24 h on 3T3-L1 cells was analyzed on the 12 th day after differentiation. Results Catechins significantly inhibited 3T3-L1 cells proliferation. Catechins and caffeine combination remarkably decreased TG accumulation after differentiation of 3T3-L1 cells, and the higher caffeine concentration was better when combined with the same catechins dose. Caffeine significantly improved NA-induced lipolysis in mature adipocytes. Conclusion Catechins and caffeine combination might inhibit cells proliferation and TG accumulation in 3T3-L1 cells. Caffeine promotes hormone-induced lipolysis in adipocytes.

关 键 词：3T3-L1细胞 脂肪细胞增殖 儿茶素 咖啡碱 脂肪代谢 

分 类 号：R151[医药卫生—营养与食品卫生学]