高分辨热裂解气相色谱/质谱法快速定性检测农产品中的单增李斯特菌  

Determination of Listeria Monocytogenes in Agricultural Products by-High Resolution Pyrolysis Gas Chromatography-Mass Spectrometry

作　　者：苏靖[1] 李晓晶[1] 韦琰琰[1] 柳婷[1] 郭爱玲[1] 

机构地区：[1]国家蛋品加工技术研发分析中心,华中农业大学食品科学技术学院,武汉430070

出　　处：《分析化学》2013年第2期187-192,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.3087955)资助项目

摘　　要：要在高分辨率热裂解气相色谱/质谱(HRPGC/MS)系统中采用选择离子检测法(SIM),建立了准确可靠、灵敏度高、快速简便的检测农产品中单增李斯特菌的方法。裂解条件:起始温度50℃;升温速率20℃/min,裂解室温度230℃;裂解温度650℃;裂解时间为10 s。气相色谱条件:载气为氦气;恒定流速0.9 mL/min;DB-WAXTER毛细管色谱柱;起始温度45℃;保持4 min,以6℃/min的速率上升到100℃,然后以10℃/min上升到200℃,接着以12℃/min上升至250℃,并保持25 min。分流比为50∶1。EI离子源。选择色谱保留时间19.056 min,鉴别离子m/z 54,98,用于SIM检测。通过对单增李斯特菌、2种空白农产品和2种不同农产品污染单增李斯特菌的样品检测结果表明,HRPGC/MS方法检测不同黄瓜、牛肉农产品中单增李斯特菌,均能得到很好的反映。本方法分析时间缩短,用保留时间、质谱同时定性,消除了不同种类农产品杂质的干扰,结果准确可靠,选择性和重复性好,适于快速检测农产品中的单增李斯特菌。A method was developed for the qualitative determination of Listeria monocytogenes in the agricultural products by high resolution pyrolysis gas chromatography-mass spectrometry-selected ion monitoring （HPRGC/MS-MIS） technology. The method was accurate, highly sensitive and easy to operate. The operation conditions of HRPGC/MS system were as follows： pyrolysis conditions were as follows： starting temperature was 50 ℃, heating rate was 20℃/min, the temperature of the pyrolysis room was 230℃, and pyrolysis temperature was 650℃ for 10 s. Gas chromatography was accomplished with a carrier gas of helium at a flow rate of 0.9 mL/min. The GC column was a DB-WAXTER capillary column. The GC column temperature initially was kept at 45 ℃ for 4 min, then programmed to 100 ℃ at 6 ℃/min, 200 ℃ at 10 ℃/min, 250 ℃ at 12 ℃/min and held for 25 min. The total flow rate was spilt at ratio of 50 to 1. EI was used as ion source. The retention time of 19. 056 min was selected for SIM, the ions of m/z were 54, 98. L. monocytogens, two kinds of agricultural products with and without L. monocytogens samples were evaluated by this method according to the characteristics of the selected ion of L. monocytogens. An expected result was observed using the HRPGC/ MS to detect L. monocytogens in different kinds of food, cucumber and beef. This method does not need the complex pre-treatment, the analysis time was comparatively short, and handing is easy for the operator. Retention time and mass spectra were used for qualitative analysis at the same time to eliminate the interference of different kinds of agricultural product impurities. The accurateness, good selectivity and reproducibility of the results demonstrate that HRPGC/MS is a useful method for rapid detection of L. monocytogens in agricultural products.

关 键 词：高分辨热裂解气相色谱 质谱 选择性离子监测 单增李斯特菌 农产品 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.63[轻工技术与工程—食品科学与工程]