一株产高温纤维素酶曲霉菌的发酵条件及培养基优化  

Optimization of medium composition and fermentation conditions for the production of thermophilic cellulase by Aspergillus sp.

作　　者：杨捷[1,2] 严芬[1] 叶秀云[1,2] 李仁宽[1,2] 刘志坚[1] 吕文静[1] 林娟[1,2] 

机构地区：[1]福州大学生物科学与工程学院,福建福州350116 [2]福州大学酶高效表达国家工程实验室,福建福州350002

出　　处：《福州大学学报（自然科学版）》2013年第1期115-120,共6页Journal of Fuzhou University(Natural Science Edition)

基　　金：福建省科技厅科研资助项目(2010J05073);福州大学人才基金资助项目(XRC-1032;XRC-0946);福州大学科技发展基金资助项目(2010-XQ-19);福建省发改委生物技术开发专项资助项目(闽发改高技[2011]1598号)

摘　　要：对一株产高温纤维素酶的曲霉(Aspergillus sp.)F4菌株进行培养基及发酵条件的优化.最佳产酶液体培养基配方为:稻草粉40 g·L-1,(NH4)2SO43.5 g·L-1,NaCl 2 g·L-1,KH2PO42 g·L-1,MgSO4.7H2O 0.5g·L-1,甘油0.20%,pH 5.5;优化后CMC酶活力提高16.7倍.优化后产酶条件为:接种量4%,温度37℃,摇床转速150 r.min-1,装液量80 mL/250 mL.在此条件下发酵8 d,CMC酶活力达到了12.26 U.mL-1,比优化前提高了约2倍.An AspergiUus sp. strain F4 capable of producing thermophilic cellulases was previously selected in the laboratory. The studies presented here described the optimization of medium components and process parameters for cellulase production by the strain using liquid fermentation. The effect of carbon sources, nitrogen sources, salts and initial culture pH on enzyme production was evaluated, and the best fermentation medium was found to be： 40 g ·L- rice straw powder, 3.5 g ·L- （NH4）2S04, 2 g ·L- NaC1, 2 g ·L- KH2P04, 0.5 g ·L- MgSO4 · 7H2O, 0.2% glycerine, pH 5.5, which resulted in a 16.7 - fold increase of CMCase yield. The inoculum size of 4% and culture volume of 80 mL/250 mL flask were optimum for cellulase production by strain F4. When incubated at 37 ~C with the shaking speed of 150 r·min-1 , highest CMCase activity of 12.26 U · mL-1 （with an- other 2 - fold increase） was seen after a period of 8 d.

关 键 词：纤维素酶 培养基 发酵条件 优化 

分 类 号：TQ925[轻工技术与工程—发酵工程] Q936[生物学—微生物学]