酵母蛋白酶A活性检测方法的比较  

Comparison of yeast proteinase A activity dectection methods

作　　者：宋群[1,2] 刘春凤[1,2] 李永仙[1,2] 王金晶[1,2] 李崎[1,2] 

机构地区：[1]江南大学教育部工业生物技术重点实验室,江苏无锡214122 [2]江南大学酿酒科学与工程研究室,江苏无锡214122

出　　处：《食品工业科技》2013年第6期61-64,共4页Science and Technology of Food Industry

基　　金：国家高技术研究发展计划(2012AA021303)

摘　　要：利用共振光散射技术建立了一种新的蛋白酶A活性检测方法-RLS法。从标准曲线、精密度、检测限、实际样品的测定等方面,对现有的蛋白酶A活性检测方法和RLS法进行了比较。结果显示,与其他三种方法（UV法、Lowry法、Bradford法）相比,RLS法检测限低（0.662μg/mL）,线性范围宽（1~500μg/mL）,标准曲线的线性良好（R2=0.9974）,精密度高（RSD=1.95%）,并在实际样品的检测中具有明显优势。有效性实验证明此方法的测定结果准确可靠,与荧光底物法测定结果之间的相关性系数为0.9917。A new method for proteinase A activity detection using resonance light scattering technique which was named as RLS method was established. The existing proteinase A activity detection methods and RLS method from the aspects of standard curve, precision,detection limit and the actual sample testing were compared. The result showed, RLS method had lower detection limit （0.662μg/mL）,wider linear range （1- 500μg/mL）, higher determination coefficient （R2=0.9974） and lower relative standard deviation （RSD=1.95%） than UV method,Lowry method and Bradford method. In addition,this method had obvious advantages in detection of actual samples. Validation test proved that RLS method was accurate and reliable,and the determination coefficient between this method and fluorescent substrate method was 0.9917.

关 键 词：蛋白酶A 酶活检测 RLS法 检测限 

分 类 号：TS262.5[轻工技术与工程—发酵工程]