白叶单枞黑茶降血糖活性成分的高速逆流色谱分离  

Separation of bioactive compounds from dark tea prepared from Camellia sinensis var. baiye dancong using HSCCC target-guided by α-glycosidase inhibitory activity

作　　者：叶琼仙[1] 尹胜[1] 周盈利[1] 苗爱清[2] 李绮汶[1] 刘静[1] 朱龙平[1] LESLIE Brown 王冬梅[1] 

机构地区：[1]中山大学药学院,广东广州510006 [2]广东省农业科学院茶叶研究所,广东英德513000 [3]AECS-QuikPrep Ltd,英国南威尔士

出　　处：《食品工业科技》2013年第6期85-87,107,共4页Science and Technology of Food Industry

基　　金：广东省自然科学基金(9151064001000038);广东省大学生创新性实验计划课题(1055811179);中山大学医科学生业余科研基金项目([2012]83号)

摘　　要：采用白叶单枞黑茶的60%乙醇提取物,在α-葡萄糖苷酶抑制活性的导向下,经D101大孔吸附树脂柱层析获得活性流分,进一步采用高速逆流色谱法(HSCCC)进行分离纯化研究。结果显示,黑茶60%乙醇提取物具有较显著的α-葡萄糖苷酶抑制活性,经D101柱层析所得的流分D-6、D-7及D-8在25μg/mL浓度下具有较黑茶60%乙醇提取物强的酶抑制活性,酶活性抑制率分别为69.31%、77.53%及75.23%。对活性流分D-7采用溶剂系统:乙酸乙酯-正丁醇-甲醇-水-乙酸(4∶1∶1∶4∶0.1,v/v)进行了HSCCC分离,得到化合物1、2,纯度均大于95%,经结构鉴定确定二者分别为牡荆苷、异牡荆苷。a-glycosidase inhibitors in dark tea were studied systematically by the method of a combination of extraction with 60% ethanol,fractionation on D101 column,and further purification by HSCCC ordinally. The result showed that fractions D-6, D-7 and D-8 obtained by D101 column chromatography indicated higher inhibitory activity than 60% ethanol extract of dark tea in the concentration 25μg/mL, inhibition were 69.31%, 77.53% and 75.23%,respectively. Fraction D-7 was selected to further separation by HSCCC with solvent system ethyl acetate-n-buthanol-methanol-water-acetic acid （4：1 ： 1：4：0.1, v/v）. Compound 1 （vitexin） and compound 2 （isovitexin） were obtained by one step HSCCC separation with purities higher than 95%, respectively.

关 键 词：黑茶 白叶单枞 Α-葡萄糖苷酶 高速逆流色谱 

分 类 号：TS272[农业科学—茶叶生产加工]