光果甘草叶总黄酮测定方法  

Determination of Total Flavonoids in Leaves of Glycyrrhiza glabra L. by Spectrophotometry

作　　者：董怡[1] 林恋竹[1] 赵谋明[1] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640

出　　处：《食品科学》2013年第6期195-198,共4页Food Science

基　　金：广东省科技计划项目(2009B011300004);广东省产学研项目(2010A090200041)

摘　　要：选用4种常用的黄酮显色方法,通过波长扫描分别确定3种标准对照品(芦丁、槲皮素、甘草苷)及新疆光果甘草叶80%乙醇提取物在各显色方法下的两个吸收波长,并分别建立标准曲线。按照建立标准曲线的方法测定新疆光果甘草叶80%乙醇提取物中总黄酮含量,并对各测定方法的精密度、稳定性、重现性及加样回收率进行测定。通过对比实验结果最终确定光果甘草叶总黄酮测定方法为:以槲皮素为对照品,AlCl3-CH4O显色法,检测波长为314nm;在此条件下,该方法的精密度、重现性、稳定性及加样回收率的相对标准偏差(RSD)分别为0.92%、2.15%、0.87%和(105.02±1.11)%。Four coloration methods were applied for the determination of total flavonoids in leaves of Glycyrrhiza glabra L. from Xinjiang. Two absorption wavelengths were selected in terms of wavelength scanning of 80% ethanol extract from the leaves and standard controls including rutin, quercetin and liquiritin. Standard curves for three standard controls by four coloration methods were established. The accuracy, stability, repeatability and recovery rates of four coloration methods were also determined. The content of total flavonoids were determined using A1C13-CH4O coloration at 314 nm and quercetin as the control. The RSD for accuracy, stability, repeatability and recovery rate were 0.92%, 0.87%, 2.15% and （105.02±1.11）%, respectively.

关 键 词：光果甘草 总黄酮 槲皮素 

分 类 号：TS202.3[轻工技术与工程—食品科学]