耐镉肠杆菌的分子鉴定和抗性分析  

Molecular identification and Cd～(2+) resistance analysis of Enterobacter sp.

作　　者：侯进慧[1] 李同祥[1] 陈薪宇[1] 王圣旺[1] 赵明涛[1] 

机构地区：[1]徐州工程学院食品(生物)工程学院,江苏徐州221008

出　　处：《食品工业科技》2013年第7期149-152,共4页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(31270577);江苏省科技创新与成果转化(重大科技支撑与自主创新)专项引导资金(BE2011644);江苏省高校自然科学研究项目(12KJD550003);徐州工程学院校级培育科研项目(XKY2011110);江苏省大学生实践创新训练计划项目(2012-2292)

摘　　要：分离到一株耐镉肠杆菌NP23,对其进行了分子鉴定和抗性研究,分析菌株在不同镉浓度条件下菌体的生长状况以及对Fe^(2+),Cu^(2+),Mn^(2+),Zn^(2+)等重金属抗性,利用SDS法进行质粒消除分析。结果显示,菌株NP23是Enterobacter sp,高浓度的镉使该菌株生长延滞期变长,进入对数期后的生长量相对较低,采用SDS法进行质粒消除,初步确定NP23的镉抗性基因在基因组上,NP23对所分析的四种重金属都有一定抗性,其中对Zn^(2+)和Mn^(2+)的抗性较强,在3200mg/L以上。In the paper, a cadmium-resistance Enterobacter NP23 was separated, and its molecular identification and cadmium resistance were analyzed.Cell growth curve was studied in the liquid medium of different cadmium concentration,Fe2＋, Cu2., Mn2＋ and Zn2＋ resistance of NP23 was analyzed, and plasmid elimination with SDS method was studied.The results showed that the strain NP23 was an Enterobacter sp.The results revealed that lag phase was extended and biomass in logarithmic phase was relatively low at high cadmium concentration.Piasmid elimination with SDS method showed NP23 cadmium resistance gene is located in the genome.Analysis of Fe2＋ , Cu2＋ ,Mn2＋ and Zn2＋ resistance of NP23 showed that the strain had a certain resistance of these four heavy metals,among which the strain had a high MIC of Zn2＋ and Mn2＋ ,3200mg/L or more.

关 键 词：重金属抗性 镉 肠杆菌 质粒消除 

分 类 号：TS201.3[轻工技术与工程—食品科学]