多种生姜有效成分联合提取工艺  

The optimum extraction process of ginger various effective components

作　　者：高晓东[1] 王秋英 乔旭光[1] 李锋[1] 唐晓珍[1] 

机构地区：[1]山东农业大学食品科学与工程学院 [2]山东省科学技术干部学校,山东济南250100

出　　处：《食品与发酵工业》2013年第1期203-207,共5页Food and Fermentation Industries

基　　金：国家科技支撑计划子课题"生姜功能成分高效分离关键技术研究与改善神经细胞功能生姜活性成分的制备关键技术与应用"(2012BAD33B07);国家农业科技成果转化资金项目"生姜蛋白酶高效分离技术及产业化"(2011GB2C600022);山东省科技攻关项目"生姜多种功能性成分同步高效分离技术研究与中试"(2012GGA12030);泰安市科技发展项目"生姜高附加值功能性食品综合开发"(20113075)

摘　　要：为了研究生姜中有效成分姜辣素、姜黄素以及生姜蛋白酶同步提取的方法,文中使用乙醇溶剂法结合旋蒸确定了生姜中3种有效成分同步提取的工艺条件,即生姜与无水乙醇料液比1∶3(g∶mL),生姜蛋白酶提取中缓冲液pH值为6.5,冰浴时间7 h;姜辣素、姜黄素旋转蒸发最佳温度为60℃,转速为16 r/min。在此优化条件下,生姜中姜辣素、姜黄素的提取率分别为0.3143%、0.3889%;生姜蛋白酶的得率为1.376%,比活力达到4 465 U/mg。此工艺能够同步提取姜辣素、姜黄素以及生姜蛋白酶3种有效成分,减少了原材料的消耗,降低生产成本,更适合产业化生产。In order to study the effective extraction of ginger oleoresin,ginger protease and curcumin simultaneous extraction,ethanol solvent combined with rotary evaporation was studied.The ratio of ginger and ethanol liquid ratio was 1∶ 3 g∶ mL）,extraction buffer pH 6.5,ice bath time 7h;for the ginger oleoresin and curcumin rotary evaporation,the optimum temperature was 60℃,the rotating speed was 16 r/min.Under the above optimized conditions,gingerol in ginger,curcumin extraction rate was 0.314 3% and 0.388 9%;ginger protease ratio was 1.376%,specific enzymatic activity reached to 4 465 U/mg.This process can simultaneously extract gingerol,curcumin and ginger protease three effective components,reduce the consumption of raw materials,the production cost,and therefore more suitable for industrial production.

关 键 词：生姜 有效成分 生姜蛋白酶 姜黄素 姜辣素 联合提取 

分 类 号：TS225.1[轻工技术与工程—粮食、油脂及植物蛋白工程]