红松松塔多糖三氯乙酸法脱蛋白工艺的研究  

Deproteinization technology of polysaccharides from Pinus koraiensis with trichloroacetic acid method

作　　者：徐韧博[1] 邹攀[1] 杨鑫[1,2] 蒙琦[1] 黄微薇[1] 胡兴龙[1] 董爱军[1] 王静[2] 

机构地区：[1]哈尔滨工业大学食品科学与工程学院,哈尔滨150090 [2]中国农业科学院农业质量标准与检测技术研究所农产品质量与安全重点实验室,北京

出　　处：《中国甜菜糖业》2013年第1期5-8,共4页China Beet & Sugar

基　　金：国家自然科学基金项目支持（资助号：31000831）;黑龙江省青年科学基金项目支持（QC08C01）;第四十五批中国博士后科学基金项目支持（20090450478）;哈尔滨工业大学科研创新基金项目支持（HIT.NSRIF.2008.30）

摘　　要：采用三氯乙酸法对红松松塔多糖进行脱蛋白工艺研究。以多糖保留率和蛋白质脱除率为指标，利用单因素和正交试验研究了多糖溶液与三氯乙酸的比例、震荡时间和三氯乙酸浓度对松塔粗多糖脱蛋白工艺的影响。研究结果表明，三氯乙酸法最佳脱蛋白工艺条件为多糖溶液与三氯乙酸的比例为1：3、震荡时间20min及三氯乙酸浓度6％，脱蛋白率达到72．41％。本研究为红松松塔多糖的进一步纯化提供了依据，为松塔的开发利用奠定理论基础。The deproteinization - technology of polysaccharides in pine Cone from Pinus koraiensis with TCA has been studied in this paper. Based on polysaccharide retention rate and deproteinization rate as evaluating indica- tors, the effects of sample solution/TCA ratio, shaking time and TCA concentration were studied. The experiment conditions were optimingized by orthogonal design, and the optimum conditions were obtained as follows： sample solution/TCA ratio was 1 ： 3 （ v/v ）, deproteinization rate was 72.41%. This study of Pinus koraiensis. shaking time was 20 min and TCA concentration was 6%. The provided the theoretical basis for the production of polysaccharide

关 键 词：红松 松塔 多糖 脱蛋白 三氯乙酸法 

分 类 号：TS245.9[轻工技术与工程—制糖工程]