检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:王艳梅[1] 万全[2] 赖年悦 林琳[1] 叶应旺[1] 姜绍通[1] 陆剑锋[1]
机构地区:[1]合肥工业大学生物与食品工程学院,安徽合肥230009 [2]安徽农业大学动物科技学院,安徽合肥230036 [3]合肥市畜牧水产技术推广中心,安徽合肥230022
出 处:《水产学报》2013年第4期622-630,共9页Journal of Fisheries of China
基 金:国家星火计划重点项目(2012GA710002);安徽省115产业创新团队计划资助
摘 要:龟类不仅营养价值高,而且具有潜在的药用价值,通过研究龟肉酶解产物的功能特性,可以科学认识其营养保健功能。以黄缘盒龟肉为原料,羟自由基清除率为指标,碱性蛋白酶为水解酶,通过正交试验L9(34)得到制备抗氧化肽的最佳酶解条件为pH值8.0、酶解温度55℃、料液比1.5∶20 g/mL、加酶量(酶/底物,E/S)3.0%、酶解时间3 h,酶解液对羟自由基清除率达到82.08%。酶解产物体外抗氧化活性的结果表明,酶解产物对羟自由基、超氧阴离子自由基、DPPH自由基和过氧化氢均有较好的清除作用,还具一定的还原能力、亚铁离子螯合能力和亚油酸自氧化抑制能力。总体而言,黄缘盒龟酶解产物在不同的体外抗氧化体系中均表现出一定的抗氧化效果,具有良好的开发利用前景。Antioxidants are known to be beneficial to human health as they may protect the body against damage by reactive oxygen species,which can attack DNA,protein and membrane lipids and play a critical role in a variety of disease such as cancer,diabetes,cardiovascular disease and Alzheimer's disease.However,there is much evidence that the use of natural antioxidants in foodstuffs is more attractive because of the potential health hazards associated with synthetic antioxidants in vivo.Turtle not only has a high nutritional value,but also has a potential medicinal value.In order to better know turtle's nutrition and healthy function,the functional properties of enzymatic hydrolysates from yellow-margined box turtle(Cistoclemmys flavomarginata)muscle were investigated in this research.An enzymatic treatment procedure was devised to make the preparation of antioxidant peptide.By using a L9(34)orthogonal array design,the optimum conditions for hydrolyzing yellow-margined box turtle muscle with Alcalase to obtain antioxidant peptides with the highest hydroxyl radical scavenging activity were as follows:pH 8.0,temperature 55 ℃,material-to-liquid ratio 1.5∶ 20 g/mL,enzyme-to-substrate ratio 3.0% and reaction time 3 h.Under the above conditions,the scavenging rate of hydroxyl radical was 82.08%.Subsequently,the antioxidant properties of yellow-margined box turtle hydrolysates were determined in vitro.The results showed that the enzymatic hydrolysates had good scavenging abilities on hydroxyl radical(·OH),superoxide anion radical(O2-·),α,α-diphenyl-β-picrylhydrazyl radical(DPPH·)and hydrogen peroxide(H2O2),and they also had certain reducing power,ferrous ion chelating capacity and the inhibition of linoleic acid autoxidation.In general,the yellow-margined box turtle hydrolysates showed certain antioxidant activities in different systems in vitro,and they will have new and broad prospects.
分 类 号:S986.2[农业科学—捕捞与储运]
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.145