纤维素酶高产菌株产酶条件的优化  

Fermentation optimization of cellulase production from high-yield strain

作　　者：薛桥丽[1] 排玲玲[2] 胡永金[2] 金铁铃[2] 

机构地区：[1]云南农业大学图书馆,云南昆明650201 [2]云南农业大学食品科学技术学院,云南昆明650201

出　　处：《中国酿造》2013年第3期110-113,共4页China Brewing

基　　金：国家自然科学基金项目(30960308);云南省自然科学基金项目(2008ZC057M)

摘　　要：通过产酶培养基及产酶条件的优化,确定产纤维素酶菌株N-57的最佳产酶条件。利用液体发酵培养法探讨培养基起始pH值、培养温度、碳源、氮源对菌株N-57产酶的影响。在单因素试验的基础上,通过Box-Behnken响应曲面法筛选出产CMCase的最佳发酵条件:pH值为6;稻草粉浓度为0.30%;(NH4)2SO4浓度为0.30%。在最佳条件下培养,该菌株的CMCase的酶活达到459.54U。The best conditions for producing cellulose of the high-yield strains N-57 were determined by optimizing fermentation medium and the condition in this paper. Different conditions including growth temperature, initial pH value, carbon source and nitrogen source were studied with liq- uid-state fermentation. On the basis of single factor test, the best conditions for producing enzyme by orthogonal analysis was pH value 6, straw pow- der concentration 0.30%, （NH~ 2SO4 concentration 0.30%. Under the optimum condition, the activity of CMCase was up to 459.54U.

关 键 词：纤维素酶 产酶条件优化 酶活性 响应曲面法 

分 类 号：TQ925[轻工技术与工程—发酵工程]