ERIC-PCR技术对单增李斯特菌的溯源分析  

Biotracing the source of Listeria monocytogenes strains by enterobacterial repetitive intergenic consensus sequence-based PCR

作　　者：刘海泉[1,2,3] 朱颖[1,2,3] 姜文洁[1,2,3] 孙晓红[1,2,3] 吴启华 潘迎捷[1,2,3] 赵勇[1,2,3] 

机构地区：[1]上海海洋大学食品学院,上海201306 [2]农业部水产品贮藏保鲜质量安全风险评估实验室(上海),上海201306 [3]上海水产品加工及贮藏工程技术研究中心,上海201306 [4]美国缅因大学食品科学与人类营养系,美国缅因州044695735

出　　处：《食品工业科技》2013年第8期49-51,60,共4页Science and Technology of Food Industry

基　　金：上海市科学技术委员会部分地方院校能力建设项目(11310501100);上海市科学技术委员会科技创新行动计划项目(12391901300);上海市科学技术委员会工程中心建设项目(11DZ2280300)

摘　　要：以质控菌株ATCC 19115为对照,采用ERIC-PCR方法对从三个市场猪肉样品分离到的17株单增李斯特菌(Listeria monocytogenes)进行了基因分型,探讨了单增李斯特菌基因型与区域分布及流行性的关联性。结果表明,17株单增李斯特菌菌株可分为六个主要基因类群,其中Ⅳ型菌株最多,为主要污染类群,而这些菌株来自于市场三;市场一和市场二分离到的菌株主要分别为Ⅰ型和Ⅳ型。因此,ERIC-PCR方法适用于对单增李斯特菌的溯源分析和流行病学调查,具有简单、方便、快捷、准确的特点。Enterobacterial repetitive intergenic consensus sequence （ERIC）-PCR was used to genotype 17 strains of Listeria monocytogenes,which were isolated from pork samples of the three market,and we investigated the correlation between the genotype, regional distribution and prevalence among L. rnonocytogenes strains. L. monocytogenes ATCC 19115 was used as positive control. The result showed that 17 isolates were identified as six special genotypes,and genotype IV was the dominant one as the main pollution group,which were isolated from the third market. The strains isolated from the first and second market were genotype Ⅰ and genotype Ⅳ, respcetively. The result suggested that ERIC-PCR was suitable to investigate the biotracing of L. monocytogenes and it was a more rapid,eff;cient, and accurate molecular typing method than traditional serotyping methods. K

关 键 词：单增李斯特菌 ERIC—PCR 基因分型 溯源 

分 类 号：TS201.2[轻工技术与工程—食品科学]