嗜酸乳杆菌转化菜籽油生成共轭亚油酸条件的优化  

Optimization of the conditions for conversion of colza oil into conjugated linoleic acid by Lactobacillus acidophilus

作　　者：李垚[1] 徐尔尼[1] 杨欣[1] 胡冰彬[1] 

机构地区：[1]南昌大学生命科学与食品工程学院食品科学与技术国家重点实验室,江西南昌330047

出　　处：《食品与发酵工业》2013年第3期32-37,共6页Food and Fermentation Industries

基　　金：江西省科技厅科技支撑项目(20111BBF60024)

摘　　要：文中对嗜酸乳杆菌静息细胞转化菜籽油生成CLA工艺进行了优化研究,通过单因素试验,确定了最适转化液介质、介质浓度、pH、温度、转化时间和细胞浓度,同时,研究了不同金属离子对亚油酸异构酶活性的影响。在该基础上,利用Box-Benhnken中心组合设计和响应面法对转化时间、pH、温度、细胞浓度进行了工艺优化分析,确定了在菜籽油浓度为7.5 mg/mL、脂肪酶的加入量为2 mg/30 mL时,以0.1 mol/L柠檬酸-柠檬酸钠缓冲液为转化液介质条件下,最优转化工艺条件是时间25 h,pH6.5,温度36℃,细胞浓度40 mg/mL,经优化后CLA的生成量可以达到230.12±7.52μg/mL。In this paper, we focused on research of optimization conditions to convert co｜za oil into conjugated linoleic acid by Lactobacillus acidophilus. Through the single factor experiment, we determined the optimal reaction medium, medium concentration, pH, temperature, time and cell concentration. At the same time, the effect of differ- ent metal ions on linoleic acid isomerase activity was studied. On this basis, using Box-Benhnken central composite design and response surface method, we optimized and analyzed the technological design which consisted of time, pH, temperature, and cell concentration. When colza oil concentration was 7.5 mg/mL and the amount of lipase was 2mg/30mL, the optimal conversion process was as follows： time 25h, pH 6.5, temperature 36℃ , cell concentration 40mg/mL, reaction medium was the 0.1 mol/L citric acid-sodium citrate buffer solution. The optimal CLA yield could reach（230.12 ＋7.52）μ g/mL.

关 键 词：嗜酸乳杆菌 转化 菜籽油 共轭亚油酸 

分 类 号：TS221[轻工技术与工程—粮食、油脂及植物蛋白工程]