大米源硒代多肽的酶法制备及其抗氧化活性的研究  

The Study of Preparation of Peptides Derived from Se-Enriched Rice Endosperm by Enzymatic Hydrolysis and Their Antioxidant Activity

作　　者：邱伟芬[1] 罗佩竹[1] 方勇[1] 杨文建[1] 马宁[1] 陈曦[1] 汤晓智[1] 胡秋辉[1] 

机构地区：[1]南京财经大学食品科学与工程学院江苏省粮油品质控制及深加工技术重点实验室,南京210023

出　　处：《中国粮油学报》2013年第4期1-7,共7页Journal of the Chinese Cereals and Oils Association

基　　金：国家自然科学基金(31101247);江苏省教育厅青蓝工程项目资助

摘　　要：以富硒碎米为原料,研究了大米含硒蛋白提取条件、硒代多肽的酶法制备及其抗氧化活性。结果表明:NaOH浓度为0.07 mol/L、料液比1∶20(m/V)、40℃下提取3 h时可获得蛋白含量、硒含量分别为80.18%和0.583 mg/kg的大米含硒蛋白。相对于木瓜蛋白酶和胃蛋白酶,胰蛋白酶水解大米含硒蛋白可获得具有更高抗氧化活性的多肽。在胰蛋白酶与底物浓度比1∶10,底物质量浓度60 g/L,pH 9.0,温度50℃的最佳酶解工艺条件下,所得大米硒代多肽的DPPH自由基清除率可达78.19%。因此,碱法提取是一种比较有效的大米含硒蛋白的提取方法,胰蛋白酶水解可获得较高抗氧化活性的大米硒代多肽。The extraction condition of Se-containing proteins, enzymatic preparation and antioxidant activity of seleno-peptides derived from Se-enriched rice endosperm was investigated. The results showed that maximum extractability was reached when rice endosperm was extracted at 40 ℃ for 3 h with using 1：20（w/v）, 0.07 mol/L NaOH. Under this condition, the protein and selenium contents of Se-containing proteins were 80.18% and 0.583 mg/kg, respectively. Trypsin appears to be the most desirable for producing high antioxidant peptides from Se-containing rice proteins. The optimum enzymatic hydrolysis condition was selected at an enzyme than substrate concentration of 10%, an substrate concentration of 6%, pH 9.0 and a temperature of 50℃. Under the optimum condition, the DPPH free radical scavenging rates of prepared seleno-peptides was raised up to 78.19%. In conclusion, alkali extraction was one of the most effective methods to extract Se-containing proteins from rice endosperm, and seleno-peptides prepared by trypsin hydrolysis exhibited higher antioxidant activity.

关 键 词：大米蛋白 硒 多肽 酶水解 抗氧化活性 

分 类 号：TS210.1[轻工技术与工程—粮食、油脂及植物蛋白工程]