川渝地区野生大茶树儿茶素和咖啡碱含量比较分析  

Comparative Analysis of Catechin and Caffeine Content of Wild Tea Plant in Sichuan-Chongqing Region

作　　者：张凯[1] 丁阳平[1,2,3] 杨坚[1,2,3] 

机构地区：[1]西南大学食品科学学院,重庆400715 [2]重庆市农产品加工重点实验室,重庆400715 [3]西南大学茶叶研究所,重庆400715

出　　处：《应用与环境生物学报》2013年第2期379-382,共4页Chinese Journal of Applied and Environmental Biology

基　　金：西南大学基本科研业务费专项资金项目(XDJK2010C075);西南大学博士基金项目(SWU111040);重庆市自然科学基金项目(CSTC;2010BB1134)资助~~

摘　　要：以儿茶素类和咖啡碱含量为筛选指标,对川渝地区野生大茶树资源进行调查研究.采用高效液相色谱法检测茶样中儿茶素类和咖啡碱含量.结果显示:川渝地区野生大茶树儿茶素总量的变幅为8.028%-17.686%;酯型儿茶素含量的变幅为1.108%-15.454%,其中表没食子儿茶素没食子酸酯(EGCG)含量的变幅为0.344%-13.248%;非酯型儿茶素含量的变幅为1.877%-7.496%;咖啡碱含量的变幅为0.323%-6.252%,其中黄山苦茶的咖啡碱含量只有0.323%.发现了4份高EGCG茶树资源,其EGCG含量大于7%;1份超高含量EGCG茶树资源,其EGCG含量大于13%;2份高酯型儿茶素/儿茶素总量之比的茶树资源,其比值大于80%;2份高咖啡碱茶树资源,其咖啡碱含量均大于5%;1份低咖啡碱茶树资源,咖啡碱含量只有0.323%,是一种天然的低咖啡碱茶树资源.This study aimed to investigate and screen wild tea plant （Camellia sinensis （L.） O. Kuntze） resources in the Sichuan-Chongqing region, with the contents of catechins and caffeine as indicators. The contents of catechin and caffeine in tea samples were determined by HPLC. The result was as follows： the total catechin content ranged from 8.028% to 17.686%; the ester catechin content ranged from 1.108% to 15.454%, with the EGCG content ranging from 0.344% to 13.248%; the non-esterified catechin content ranged from 1.877% to 7.496%; the caffeine content ranged from 0.323% to 6.252%. Among those tea samples, the content of caffeine in Huangshan kucha was only 0.323%. This study found 4 EGCG-rich tea samples, with EGCG content more than 7%; 1 sample with even higher EGCG content of more than 13%; 2 samples with the ratio of ester-catechin contents to total catechin content exceeding 80%; 2 caffeine-rich tea samples with the caffeine content higher than 5%; and 1 tea sample with caffeine contents as low as 0.323%, a natural low-caffeine tea resource. Fig 1, Ref 22

关 键 词：野生大茶树 儿茶素 咖啡碱 高效液相色谱 

分 类 号：S571.1[农业科学—茶叶生产加工]