Protective effect of Jiaweibugan decoction against diabetic peripheral neuropathy  

作　　者：Yu Wang Zeqi Chen Renqun Ye Yulei He Yuhong Li Xinjian Qiu 

机构地区：[1]Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University [2]Department of Internal Medicine, Chinese Medical Hospital in Bao'an District of Shenzhen City

出　　处：《Neural Regeneration Research》2013年第12期1113-1121,共9页中国神经再生研究（英文版）

基　　金：supported by the Scientific Research Foundation of Traditional Chinese Medicine of Hunan Provincial Health Bureau,No.06202

摘　　要：Oxygen free radical damage is regarded as a direct or indirect common pathway associated with diabetic neuropathy and is the main cause of complications in peripheral neuropathies. We speculate that Jiaweibugan decoction has a significant effect in treating diabetic peripheral neuropathy through an anti-oxidative stress pathway. In this study, a diabetic rat model was established by intraperitoneal injection of streptozotocin. Rats were treated with Jiaweibugan decoction via intragastric administration. The levels of malondialdehyde and glutathione, which are indirect indexes of oxidative stress, in serum were determined using a colorimetric method. The expression levels of nuclear factor kappa B p65 mRNA and p38 mitogen-activated protein kinase, which are oxidative stress associated factors, in the dorsal root ganglion of spinal $4-6 segments were evaluated by reverse-transcriptase polymerase chain reaction and immunohistochemistry. Results showed that, Jiaweibugan decoction significantly ameliorated motor nerve conduction velocity in diabetic rats, effectively decreased malondialdehyde levels in serum and the expression of nuclear factor kappa B p65 mRNA and p38 mitogen-activated protein kinase mRNA in the dorsa root ganglion, and increased glutathione levels in serum. Therefore, our experimental findings indicate that Jiaweibugan decoction plays an anti-oxidative stress role in the diabetic peripheral neuropathy process, which has a protective effect on peripheral nerve injury.Oxygen free radical damage is regarded as a direct or indirect common pathway associated with diabetic neuropathy and is the main cause of complications in peripheral neuropathies. We speculate that Jiaweibugan decoction has a significant effect in treating diabetic peripheral neuropathy through an anti-oxidative stress pathway. In this study, a diabetic rat model was established by intraperitoneal injection of streptozotocin. Rats were treated with Jiaweibugan decoction via intragastric administration. The levels of malondialdehyde and glutathione, which are indirect indexes of oxidative stress, in serum were determined using a colorimetric method. The expression levels of nuclear factor kappa B p65 mRNA and p38 mitogen-activated protein kinase, which are oxidative stress associated factors, in the dorsal root ganglion of spinal $4-6 segments were evaluated by reverse-transcriptase polymerase chain reaction and immunohistochemistry. Results showed that, Jiaweibugan decoction significantly ameliorated motor nerve conduction velocity in diabetic rats, effectively decreased malondialdehyde levels in serum and the expression of nuclear factor kappa B p65 mRNA and p38 mitogen-activated protein kinase mRNA in the dorsa root ganglion, and increased glutathione levels in serum. Therefore, our experimental findings indicate that Jiaweibugan decoction plays an anti-oxidative stress role in the diabetic peripheral neuropathy process, which has a protective effect on peripheral nerve injury.

关 键 词：neural regeneration traditional Chinese medicine peripheral nerve injury Jiaweibugan decoctiondiabetic peripheral neuropathy MALONDIALDEHYDE GLUTATHIONE nuclear factor kappa B p65 p38mitogen-activated protein kinase oxidative stress TAURINE STREPTOZOTOCIN oxygen free radicaldamage grants-supported paper NEUROREGENERATION 

分 类 号：R742.1[医药卫生—神经病学与精神病学]