抗氧化肽VHHH来源初步研究  

Source of antioxidant peptides VHHH

作　　者：胡立明[1,2] 曹晓虹[3] 顾振新[1] 

机构地区：[1]南京农业大学食品科技学院,南京210095 [2]徐州空军学院,徐州221006 [3]北方民族大学生物科学与工程学院,银川750021

出　　处：《食品科技》2013年第5期255-259,共5页Food Science and Technology

摘　　要：对来自于麦胚自身水解的抗氧化肽VHHH来源进行了初步研究。利用麦胚的粗酶液分别等量水解相同的蛋白,然后再比较VHHH的含量。结果显示:麦胚中的4种可溶性蛋白的含量分别为:清蛋白2.88±0.27%、球蛋白1.10±0.15%、醇溶蛋白0.17±0.03%、谷蛋白0.90±0.08%;在保留时间11.138min处,麦胚蛋白的酸水解液中没有发现VHHH,这表明这种肽不是来自于麦胚蛋白的酸水解;VHHH在麦胚球蛋白的水解物中含量最高,达到4.33%,其次是麦胚清蛋白(4.05%),再次是麦胚醇溶蛋白和麦胚谷蛋白(分别为0.57%和0.48%)。这表明VHHH蛋白源主要来自于麦胚球蛋白,其次来自于麦胚清蛋白。An antioxidant peptide of VHHH from wheat germ by its autolysis reaction was studied on its trace.The same amount of soluble proteins was hydrolyzed by its crude enzyme,and then VHHH contents were compared between hydrolyses by HPLC.The results showed that albumin accounted for 2.88±0.27%,globulin 1.10±0.15%,ethanol-soluble protein 0.17±0.03% and grain protein 0.90±0.08%,during the four wheat germ soluble proteins.In the retention time of 11.138 min,the hydrolysis did not find VHHH,indicating that this peptide was not derived from wheat germ protein acid hydrolysis.Globulin hydrolyses had at the highest concentrations of VHHH （4.33%）,followed by albumin （4.05%）,ethanolsoluble protein and gluten （0.57% and 0.48%,respectively）.This indicated that VHHH protein mainly came from wheat germ globulin,then albumin.

关 键 词：麦胚 抗氧化肽 可溶性蛋白 

分 类 号：TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程]